PMID- 34443935
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 11
IP  - 8
DP  - 2021 Aug 19
TI  - Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, 
      Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in 
      Human Chondrocytes.
LID - 10.3390/nano11082105 [doi]
LID - 2105
AB  - Recently, graphene and its derivatives have been extensively investigated for 
      their interesting properties in many biomedical fields, including tissue 
      engineering and regenerative medicine. Nonetheless, graphene oxide (GO) and 
      reduced GO (rGO) are still under investigation for improving their dispersibility 
      in aqueous solutions and their safety in different cell types. This work explores 
      the interaction of GO and rGO with different polymeric dispersants, such as 
      glycol chitosan (GC), propylene glycol alginate (PGA), and polydopamine (PDA), 
      and their effects on human chondrocytes. GO was synthesized using Hummer's 
      method, followed by a sonication-assisted liquid-phase exfoliation (LPE) process, 
      drying, and thermal reduction to obtain rGO. The flakes of GO and rGO exhibited 
      an average lateral size of 8.8 +/- 4.6 and 18.3 +/- 8.5 microm, respectively. Their 
      dispersibility and colloidal stability were investigated in the presence of the 
      polymeric surfactants, resulting in an improvement in the suspension stability in 
      terms of average size and polydispersity index over 1 h, in particular for PDA. 
      Furthermore, cytotoxic effects induced by coated and uncoated GO and rGO on human 
      chondrocytes at different concentrations (12.5, 25, 50 and 100 microg/mL) were 
      assessed through LDH assay. Results showed a concentration-dependent response, 
      and the presence of PGA contributed to statistically decreasing the difference in 
      the LDH activity with respect to the control. These results open the way to a 
      potentially safer use of these nanomaterials in the fields of cartilage tissue 
      engineering and regenerative medicine.
FAU - Vannozzi, Lorenzo
AU  - Vannozzi L
AUID- ORCID: 0000-0003-3525-5073
AD  - The BioRobotics Institute, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
AD  - Department of Excellence in Robotics & AI, Scuola Superiore Sant'Anna, 56127 
      Pisa, Italy.
FAU - Catalano, Enrico
AU  - Catalano E
AD  - The BioRobotics Institute, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
AD  - Department of Excellence in Robotics & AI, Scuola Superiore Sant'Anna, 56127 
      Pisa, Italy.
FAU - Telkhozhayeva, Madina
AU  - Telkhozhayeva M
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Teblum, Eti
AU  - Teblum E
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Yarmolenko, Alina
AU  - Yarmolenko A
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Avraham, Efrat Shawat
AU  - Avraham ES
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Konar, Rajashree
AU  - Konar R
AUID- ORCID: 0000-0003-2938-5541
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Nessim, Gilbert Daniel
AU  - Nessim GD
AD  - Department of Chemistry and Institute of Nanotechnology, Bar-Ilan University, 
      Ramat Gan 52900, Israel.
FAU - Ricotti, Leonardo
AU  - Ricotti L
AD  - The BioRobotics Institute, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
AD  - Department of Excellence in Robotics & AI, Scuola Superiore Sant'Anna, 56127 
      Pisa, Italy.
LA  - eng
GR  - 814413/Horizon 2020 Framework Programme/
PT  - Journal Article
DEP - 20210819
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC8399274
OTO - NOTNLM
OT  - LDH
OT  - cytotoxicity
OT  - glycol chitosan
OT  - graphene oxide
OT  - human chondrocytes
OT  - polydispersity index
OT  - polydopamine
OT  - propylene glycol alginate
OT  - reduced graphene oxide
COIS- The authors have no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/19
CRDT- 2021/08/27 01:18
PHST- 2021/07/27 00:00 [received]
PHST- 2021/08/12 00:00 [revised]
PHST- 2021/08/12 00:00 [accepted]
PHST- 2021/08/27 01:18 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/19 00:00 [pmc-release]
AID - nano11082105 [pii]
AID - nanomaterials-11-02105 [pii]
AID - 10.3390/nano11082105 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2021 Aug 19;11(8):2105. doi: 10.3390/nano11082105.