PMID- 34445245
OWN - NLM
STAT- MEDLINE
DCOM- 20210915
LR  - 20210915
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 16
DP  - 2021 Aug 9
TI  - Chromosomal Characterization of Tripidium arundinaceum Revealed by Oligo-FISH.
LID - 10.3390/ijms22168539 [doi]
LID - 8539
AB  - Sugarcane is of important economic value for producing sugar and bioethanol. 
      Tripidium arundinaceum (old name: Erianthus arundinaceum) is an 
      intergeneric wild species of sugarcane that has desirable resistance traits for 
      improving sugarcane varieties. However, the scarcity of chromosome markers has 
      hindered the cytogenetic study of T. arundinaceum. Here we applied maize 
      chromosome painting probes (MCPs) to identify chromosomes in sorghum and T. 
      arundinaceum using a repeated fluorescence in situ hybridization (FISH) system. 
      Sequential FISH revealed that these MCPs can be used as reliable chromosome 
      markers for T. arundinaceum, even though T. arundinaceum has diverged from maize 
      over 18 MYs (million years). Using these MCPs, we identified T. arundinaceum 
      chromosomes based on their sequence similarity compared to sorghum and labeled 
      them 1 through 10. Then, the karyotype of T. arundinaceum was established by 
      multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA 
      are localized on chromosomes 5 and 6, respectively, in T. arundinaceum. 
      Altogether, these results represent an essential step for further cytogenetic 
      research of T. arundinaceum in sugarcane breeding.
FAU - Yu, Fan
AU  - Yu F
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Chai, Jin
AU  - Chai J
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Li, Xueting
AU  - Li X
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Yu, Zehuai
AU  - Yu Z
AD  - State Key Laboratory for Protection and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning 530004, China.
FAU - Yang, Ruiting
AU  - Yang R
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Ding, Xueer
AU  - Ding X
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Wang, Qiusong
AU  - Wang Q
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Wu, Jiayun
AU  - Wu J
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 
      510316, China.
FAU - Yang, Xiping
AU  - Yang X
AD  - State Key Laboratory for Protection and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning 530004, China.
FAU - Deng, Zuhu
AU  - Deng Z
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
AD  - State Key Laboratory for Protection and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning 530004, China.
LA  - eng
GR  - 31771863/National Natural Science Foundation of China/
GR  - 324-1122yb056/Scientific Research Foundation of Graduate School of Fujian 
      Agriculture and Forestry University/
GR  - 2019KJ104-04/Guangdong Provincial Team of Technical System Innovation for 
      Sugarcane Sisal Hemp Industry/
GR  - No. CARS-20-1-5/the project supported by China Agriculture Research System of MOF 
      and MARA/
PT  - Journal Article
DEP - 20210809
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Painting
MH  - Chromosomes, Plant/*genetics
MH  - DNA Probes/chemistry/genetics
MH  - Plant Breeding
MH  - Saccharum/*genetics
PMC - PMC8395171
OTO - NOTNLM
OT  - T. arundinaceum
OT  - chromosome identification
OT  - karyotype
OT  - maize chromosome painting probes
OT  - oligo-FISH
OT  - ribosomal DNA
OT  - sorghum
OT  - sugarcane
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/28 06:00
MHDA- 2021/09/16 06:00
PMCR- 2021/08/09
CRDT- 2021/08/27 01:23
PHST- 2021/07/03 00:00 [received]
PHST- 2021/08/06 00:00 [revised]
PHST- 2021/08/07 00:00 [accepted]
PHST- 2021/08/27 01:23 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/09/16 06:00 [medline]
PHST- 2021/08/09 00:00 [pmc-release]
AID - ijms22168539 [pii]
AID - ijms-22-08539 [pii]
AID - 10.3390/ijms22168539 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Aug 9;22(16):8539. doi: 10.3390/ijms22168539.