PMID- 34446765
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Aug 26
TI  - Differential responses of chicken monocyte-derived dendritic cells infected with 
      Salmonella Gallinarum and Salmonella Typhimurium.
PG  - 17214
LID - 10.1038/s41598-021-96527-w [doi]
LID - 17214
AB  - Salmonella enterica serovar Gallinarum is a host-restricted bacterial pathogen 
      that causes a serious systemic disease exclusively in birds of all ages. 
      Salmonella enterica serovar Typhimurium is a host-generalist serovar. Dendritic 
      cells (DCs) are key antigen-presenting cells that play an important part in 
      Salmonella host-restriction. We evaluated the differential response of chicken 
      blood monocyte-derived dendritic cells (chMoDCs) exposed to S. Gallinarum or S. 
      Typhimurium. S. Typhimurium was found to be more invasive while S. Gallinarum was 
      more cytotoxic at the early phase of infection and later showed higher resistance 
      against chMoDCs killing. S. Typhimurium promoted relatively higher upregulation 
      of costimulatory and other immune function genes on chMoDCs in comparison to S. 
      Gallinarum during early phase of infection (6 h) as analyzed by real-time PCR. 
      Both Salmonella serovars strongly upregulated the proinflammatory transcripts, 
      however, quantum was relatively narrower with S. Gallinarum. S. 
      Typhimurium-infected chMoDCs promoted relatively higher proliferation of naive 
      T-cells in comparison to S. Gallinarum as assessed by mixed lymphocyte reaction. 
      Our findings indicated that host restriction of S. Gallinarum to chicken is 
      linked with its profound ability to interfere the DCs function. Present findings 
      provide a valuable roadmap for future work aimed at improved vaccine strategies 
      against this pathogen.
CI  - (c) 2021. The Author(s).
FAU - Singh, Degpal
AU  - Singh D
AD  - Immunology Section, ICAR-Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Singh, Mithilesh
AU  - Singh M
AD  - Immunology Section, ICAR-Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India. drmithileshsingh@yahoo.com.
FAU - Chander, Vishal
AU  - Chander V
AD  - Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research 
      Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
FAU - Sharma, Gaurav Kumar
AU  - Sharma GK
AD  - Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research 
      Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
FAU - Mahawar, Manish
AU  - Mahawar M
AD  - Division of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Teeli, Aamir Salam
AU  - Teeli AS
AD  - Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, 
      05-552, Jastrzebiec, Poland.
FAU - Goswami, Tapas Kumar
AU  - Goswami TK
AD  - Immunology Section, ICAR-Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210826
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/genetics/immunology
MH  - CD40 Antigens/genetics/immunology
MH  - Chickens
MH  - Cytokines/genetics/immunology
MH  - Cytotoxicity, Immunologic/immunology
MH  - Dendritic Cells/*immunology/metabolism/microbiology
MH  - Gene Expression/immunology
MH  - Host-Pathogen Interactions/immunology
MH  - Microbial Viability/immunology
MH  - Monocytes/cytology/*immunology
MH  - Salmonella/*immunology/physiology
MH  - Salmonella typhimurium/*immunology/physiology
MH  - Species Specificity
MH  - T-Lymphocytes/immunology/metabolism
MH  - Toll-Like Receptors/genetics/immunology
PMC - PMC8390485
COIS- The authors declare no competing interests.
EDAT- 2021/08/28 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/08/26
CRDT- 2021/08/27 06:27
PHST- 2021/04/27 00:00 [received]
PHST- 2021/08/11 00:00 [accepted]
PHST- 2021/08/27 06:27 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/08/26 00:00 [pmc-release]
AID - 10.1038/s41598-021-96527-w [pii]
AID - 96527 [pii]
AID - 10.1038/s41598-021-96527-w [doi]
PST - epublish
SO  - Sci Rep. 2021 Aug 26;11(1):17214. doi: 10.1038/s41598-021-96527-w.