PMID- 34446787
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20240226
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Aug 26
TI  - Dendritic cells play no significant role in the laser-induced choroidal 
      neovascularization model.
PG  - 17254
LID - 10.1038/s41598-021-96704-x [doi]
LID - 17254
AB  - Age-related macular degeneration (AMD) is genetically associated with complement. 
      Dendritic cells (DCs) play key roles during innate and adaptive immunity, and 
      express complement components and their receptors. We investigated ocular DC 
      heterogeneity and the role of DCs in the laser-induced choroidal 
      neovascularization (CNV) model. In order to determine the function of DCs, we 
      used two models of DC deficiency: the Flt3(-/-) and Flt3l(-/-) mouse. We 
      identified three types of ocular DCs: plasmacytoid DC, classical DC-1, and 
      classical DC-2. At steady-state, classical DCs were found in the iris and choroid 
      but were not detectable in the retina. Plasmacytoid DCs existed at very low 
      levels in iris, choroid, and retina. After laser injury, the number of each DC 
      subset was up-regulated in the choroid and retina. In Flt3(-/-) mice, we found 
      reduced numbers of classical DCs at steady-state, but each DC subset equally 
      increased after laser injury between wildtype and Flt3(-/-) mice. In Flt3l(-/-) 
      mice, each DC subsets was severely reduced after laser injury. Neither Flt3(-/-) 
      or Flt3l(-/-) mice demonstrated reduced CNV area compared to wildtype mice. DCs 
      do not play any significant role during the laser-induced CNV model of 
      neovascular AMD.
CI  - (c) 2021. The Author(s).
FAU - Droho, Steven
AU  - Droho S
AD  - Department of Ophthalmology, Feinberg School of Medicine, Northwestern 
      University, Chicago, IL, USA.
FAU - Perlman, Harris
AU  - Perlman H
AD  - Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, 
      Northwestern University, Chicago, IL, USA.
FAU - Lavine, Jeremy A
AU  - Lavine JA
AD  - Department of Ophthalmology, Feinberg School of Medicine, Northwestern 
      University, Chicago, IL, USA. jeremy.lavine@northwestern.edu.
LA  - eng
GR  - P01 AG049665/AG/NIA NIH HHS/United States
GR  - R01 AR064546/AR/NIAMS NIH HHS/United States
GR  - P30 CA060553/CA/NCI NIH HHS/United States
GR  - K08 EY030923/EY/NEI NIH HHS/United States
GR  - R01 HL134375/HL/NHLBI NIH HHS/United States
GR  - UH2 AR067687/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20210826
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
RN  - EC 2.7.10.1 (Flt3 protein, mouse)
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
SB  - IM
MH  - Animals
MH  - Choroid/blood supply/*immunology
MH  - Choroidal Neovascularization/etiology/genetics/*immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Iris/blood supply/immunology
MH  - Lasers/adverse effects
MH  - Male
MH  - Membrane Proteins/genetics/*immunology/metabolism
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Retina/immunology
MH  - Visual Acuity/immunology
MH  - Wet Macular Degeneration/immunology
MH  - fms-Like Tyrosine Kinase 3/genetics/*immunology/metabolism
MH  - Mice
PMC - PMC8390527
COIS- The authors declare no competing interests.
EDAT- 2021/08/28 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/08/26
CRDT- 2021/08/27 06:33
PHST- 2021/06/08 00:00 [received]
PHST- 2021/08/12 00:00 [accepted]
PHST- 2021/08/27 06:33 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/08/26 00:00 [pmc-release]
AID - 10.1038/s41598-021-96704-x [pii]
AID - 96704 [pii]
AID - 10.1038/s41598-021-96704-x [doi]
PST - epublish
SO  - Sci Rep. 2021 Aug 26;11(1):17254. doi: 10.1038/s41598-021-96704-x.