PMID- 34447637
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220426
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 9
DP  - 2021
TI  - Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like 
      synovial cells in rheumatoid arthritis.
PG  - e11973
LID - 10.7717/peerj.11973 [doi]
LID - e11973
AB  - BACKGROUND: Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease 
      with persistent synovitis. In the present study, the impact of monocyte 
      chemoattractant protein-1 (MCP-1) was explored to determine methods for the 
      diagnosis and treatment of RA. METHODS: First, fibroblast-like synoviocytes 
      (FLSs) were obtained from a collagen-induced rat RA model. Next, 
      MCP-1-overexpression plasmid and small interfering RNA were transfected into 
      human and rat FLSs. Cell Counting Kit-8 (CCK-8), Transwell migration and flow 
      cytometry assays were used to analyze cell proliferation, migration and apoptosis 
      of FLSs following MCP-1 transfections, respectively. Furthermore, western 
      blotting was used to analyze the expression levels of p-P38, p-PI3K, PI3K, CD31, 
      VEGF, TNF-alpha and IL-beta in FLSs following MCP-1 transfection. In addition, reverse 
      transcription-quantitative PCR and ELISAs were used to analyze the expression 
      levels of C-reactive protein (CRP), estrogen receptor, MCP-1 and pentraxin-3 in 
      patients with clinical RA, followed by correlation analysis of clinical data. 
      Finally, expression validation, diagnostic and protein-protein interaction (PPI) 
      network analysis of MCP-1 were performed. RESULTS: MCP-1 promoted FLS 
      proliferation and migration, and affected the apoptosis of FLSs. In addition, the 
      expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-alpha and IL-beta were also 
      affected by MCP-1. In patients with clinical RA, the expression level of MCP-1 
      was increased. Moreover, CRP expression level was significantly up-regulated in 
      RA. Clinically, MCP-1 was strongly correlated with tender joint count, swollen 
      joint count, visual analog scale for general health and disease activity score 28 
      (DAS28)-MCP-1, and was moderately correlated with DAS28 and DAS28-CRP. PPI 
      analysis showed that MCP-1 mainly interacted with other inflammatory cytokines. 
      CONCLUSION: In conclusion, MCP-1 may play a significant regulatory role in RA, 
      and could be used as a measurement index of clinical RA activity.
CI  - (c)2021 Tong et al.
FAU - Tong, Xiang
AU  - Tong X
AD  - Department of Orthopedic Surgery, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Yu, Dongdong
AU  - Yu D
AD  - Department of Orthopedic Surgery, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Yu, Li
AU  - Yu L
AD  - Operating Room, The First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, Zhejiang, China.
FAU - Chen, Weiqian
AU  - Chen W
AD  - Department of Rheumatology, The First Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Wen, Yanling
AU  - Wen Y
AD  - Department of Rheumatology, The First Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Gu, Pengcheng
AU  - Gu P
AD  - Department of Orthopedic Surgery, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20210811
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC8364321
OTO - NOTNLM
OT  - Apoptosis
OT  - DAS28-MCP-1
OT  - Fibroblast-like synoviocytes cells
OT  - Migration
OT  - Monocyte chemoattractant protein-1
OT  - Over-expression
OT  - Proliferation
OT  - Rheumatoid arthritis
OT  - Silence
OT  - Western blotting
COIS- The authors declare there are no competing interests.
EDAT- 2021/08/28 06:00
MHDA- 2021/08/28 06:01
PMCR- 2021/08/11
CRDT- 2021/08/27 06:56
PHST- 2021/02/04 00:00 [received]
PHST- 2021/07/23 00:00 [accepted]
PHST- 2021/08/27 06:56 [entrez]
PHST- 2021/08/28 06:00 [pubmed]
PHST- 2021/08/28 06:01 [medline]
PHST- 2021/08/11 00:00 [pmc-release]
AID - 11973 [pii]
AID - 10.7717/peerj.11973 [doi]
PST - epublish
SO  - PeerJ. 2021 Aug 11;9:e11973. doi: 10.7717/peerj.11973. eCollection 2021.