PMID- 34451811
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220123
IS  - 1424-8247 (Print)
IS  - 1424-8247 (Electronic)
IS  - 1424-8247 (Linking)
VI  - 14
IP  - 8
DP  - 2021 Jul 23
TI  - Red Algal Sulfated Galactan Binds and Protects Neural Cells from HIV-1 gp120 and 
      Tat.
LID - 10.3390/ph14080714 [doi]
LID - 714
AB  - The potential neuroprotective capacity of four different sulfated glycans: 
      Botryocladia occidentalis-derived sulfated galactan (BoSG) (MW > 100 kDa), 
      Lytechinus variegatus-derived sulfated fucan (LvSF) (MW~90 kDa), high-molecular 
      weight dextran sulfate (DxS) (MW 100 kDa), and unfractionated heparin (UFH) 
      (MW~15 kDa), was assessed in response to the HIV-1 proteins, R5-tropic 
      glycoprotein 120 (gp120) and/or trans-activator of transcription (Tat), using 
      primary murine neurons co-cultured with mixed glia. Compared to control-treated 
      cells in which HIV-1 proteins alone or combined were neurotoxic, BoSG was, among 
      the four tested sulfated glycans, the only one capable of showing significant 
      concentration-dependent neuroprotection against Tat and/or gp120, alone or 
      combined. Surface plasmon resonance-based data indicate that BoSG can bind both 
      HIV-1 proteins at nM concentrations with preference for Tat (7.5 x 10(-8) M) over 
      gp120 (3.2 x 10(-7) M) as compared to UFH, which bound gp120 (8.7 x 10(-7) M) 
      over Tat (5.7 x 10(-6) M). Overall, these data support the notion that sulfated 
      glycan extracted from the red alga B. occidentalis, BoSG, can exert 
      neuroprotection against HIV-1 Tat and gp120, potentially via direct molecular 
      interactions.
FAU - Pomin, Vitor H
AU  - Pomin VH
AUID- ORCID: 0000-0002-5015-8880
AD  - Department of BioMolecular Sciences, School of Pharmacy, University of 
      Mississippi, University, MS 38677-1848, USA.
AD  - Research Institute of Pharmaceutical Sciences, University of Mississippi, 
      University, MS 38677, USA.
FAU - Mahdi, Fakhri
AU  - Mahdi F
AUID- ORCID: 0000-0003-4891-2992
AD  - Department of BioMolecular Sciences, School of Pharmacy, University of 
      Mississippi, University, MS 38677-1848, USA.
FAU - Jin, Weihua
AU  - Jin W
AUID- ORCID: 0000-0002-0551-0291
AD  - Center for Biotechnology and Interdisciplinary Studies, Department of Chemical 
      and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, 
      USA.
AD  - College of Biotechnology and Bioengineering, Zhejiang University of Technology, 
      Hangzhou 310014, China.
FAU - Zhang, Fuming
AU  - Zhang F
AUID- ORCID: 0000-0003-2803-3704
AD  - Center for Biotechnology and Interdisciplinary Studies, Department of Chemical 
      and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, 
      USA.
FAU - Linhardt, Robert J
AU  - Linhardt RJ
AUID- ORCID: 0000-0003-2219-5833
AD  - Center for Biotechnology and Interdisciplinary Studies, Department of Chemical 
      and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, 
      USA.
AD  - Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry 
      and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
FAU - Paris, Jason J
AU  - Paris JJ
AUID- ORCID: 0000-0002-5628-1134
AD  - Department of BioMolecular Sciences, School of Pharmacy, University of 
      Mississippi, University, MS 38677-1848, USA.
AD  - Research Institute of Pharmaceutical Sciences, University of Mississippi, 
      University, MS 38677, USA.
LA  - eng
GR  - 1P20GM130460-01A1-7936/NH/NIH HHS/United States
GR  - CA231074/NH/NIH HHS/United States
GR  - R00DA039791/NH/NIH HHS/United States
GR  - 1R03NS110996-01A1/NH/NIH HHS/United States
GR  - R03 NS110996/NS/NINDS NIH HHS/United States
GR  - DK111958/NH/NIH HHS/United States
GR  - P20 GM130460/GM/NIGMS NIH HHS/United States
GR  - R00 DA039791/DA/NIDA NIH HHS/United States
GR  - R01 DA052851/NH/NIH HHS/United States
GR  - R01 DA052851/DA/NIDA NIH HHS/United States
PT  - Journal Article
DEP - 20210723
PL  - Switzerland
TA  - Pharmaceuticals (Basel)
JT  - Pharmaceuticals (Basel, Switzerland)
JID - 101238453
PMC - PMC8398392
OTO - NOTNLM
OT  - HIV
OT  - glycoprotein 120
OT  - neuroprotection
OT  - sulfated glycan
OT  - surface plasmon resonance
OT  - trans-activating transcriptor
COIS- The authors declare no competing financial interest.
EDAT- 2021/08/29 06:00
MHDA- 2021/08/29 06:01
PMCR- 2021/07/23
CRDT- 2021/08/28 01:04
PHST- 2021/06/02 00:00 [received]
PHST- 2021/07/19 00:00 [revised]
PHST- 2021/07/20 00:00 [accepted]
PHST- 2021/08/28 01:04 [entrez]
PHST- 2021/08/29 06:00 [pubmed]
PHST- 2021/08/29 06:01 [medline]
PHST- 2021/07/23 00:00 [pmc-release]
AID - ph14080714 [pii]
AID - pharmaceuticals-14-00714 [pii]
AID - 10.3390/ph14080714 [doi]
PST - epublish
SO  - Pharmaceuticals (Basel). 2021 Jul 23;14(8):714. doi: 10.3390/ph14080714.