PMID- 34452623
OWN - NLM
STAT- MEDLINE
DCOM- 20211215
LR  - 20211215
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Aug 27
TI  - Anti-allergic activities of Umbelliferone against histamine- and Picryl 
      chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice.
PG  - 215
LID - 10.1186/s12906-021-03384-1 [doi]
LID - 215
AB  - BACKGROUND: The current study was aimed to investigate the anti-allergic 
      activities of the Umbelliferone (UMB) against the acute Histamine and chronic 
      Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative 
      (isolated from Angelica decursiva) found in various parts of the plants such as 
      flowers, roots and, stems isolated from the plants of Umbelliferae family. 
      METHODS: The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half 
      an h before or 2 h after the induction of allergic ear edema. The acute ear edema 
      was induced by histamine (intradermally, i.d), while the chronic ear edema was 
      induced by painting the PiCl (sensitized with the toluene) on the ear. The 
      antioxidants and oxidative stress markers were assessed. The histological changes 
      were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The 
      immunohistochemistry studies were performed to assess the expression of the 
      nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide 
      synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by 
      Tukey's test with p < 0.05 was chosen as criteria for statistical significance. 
      RESULTS: UMB treatment markedly reduced the allergic ear edema and ear weight 
      compared to the negative control. Furthermore, the UMB attenuated the oxidative 
      stress markers, while induced the antioxidants enzymes. Similarly, the UMB 
      treatment significantly attenuated the serum immunoglobulin E (IgE) level. The 
      UMB treatment markedly improved the histological parameters using H and E 
      staining and Giemsa staining. The UMB administration induced the Nrf2 expression, 
      while attenuated the iNOS expression. Furthermore, the computational analysis was 
      performed to assess the interaction of the UMB with the various protein targets 
      and to determine the mechanism of interaction with the target proteins. 
      CONCLUSION: In conclusion, the UMB treatment significantly alleviated the 
      allergic symptoms, attenuating the oxidative stress, improved the histological 
      features using in vivo and computational approaches.
CI  - (c) 2021. The Author(s).
FAU - Younas
AU  - Younas
AD  - Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of 
      Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
FAU - Khan, Ashrafullah
AU  - Khan A
AD  - Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of 
      Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
FAU - Shehzad, Omer
AU  - Shehzad O
AD  - Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan.
FAU - Seo, Eun Kyoung
AU  - Seo EK
AD  - College of Pharmacy, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, 
      Seoul, 03760, South Korea.
FAU - Onder, Alev
AU  - Onder A
AD  - Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, Ankara, 
      Turkey.
FAU - Khan, Salman
AU  - Khan S
AUID- ORCID: 0000-0002-6226-8470
AD  - Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of 
      Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan. 
      skhan@qau.edu.pk.
LA  - eng
PT  - Journal Article
DEP - 20210827
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Umbelliferones)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Ear Auricle/drug effects
MH  - Edema/chemically induced/*drug therapy
MH  - Mice
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/*pharmacology
MH  - Umbelliferones/*pharmacology
PMC - PMC8394045
OTO - NOTNLM
OT  - Antioxidant
OT  - Inflammation
OT  - Oxidative stress
OT  - Picryl chloride
OT  - Umbelliferone
COIS- The author declares no conflict of interest.
EDAT- 2021/08/29 06:00
MHDA- 2021/12/16 06:00
PMCR- 2021/08/27
CRDT- 2021/08/28 05:20
PHST- 2020/12/06 00:00 [received]
PHST- 2021/07/29 00:00 [accepted]
PHST- 2021/08/28 05:20 [entrez]
PHST- 2021/08/29 06:00 [pubmed]
PHST- 2021/12/16 06:00 [medline]
PHST- 2021/08/27 00:00 [pmc-release]
AID - 10.1186/s12906-021-03384-1 [pii]
AID - 3384 [pii]
AID - 10.1186/s12906-021-03384-1 [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2021 Aug 27;21(1):215. doi: 10.1186/s12906-021-03384-1.