PMID- 34456192
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220428
IS  - 2005-3606 (Print)
IS  - 2005-5447 (Electronic)
IS  - 2005-3606 (Linking)
VI  - 14
IP  - 4
DP  - 2021 Nov 30
TI  - Sox9 Is Crucial for Mesenchymal Stem Cells to Enhance Cutaneous Wound Healing.
PG  - 465-474
LID - 10.15283/ijsc21078 [doi]
AB  - BACKGROUND AND OBJECTIVES: Human umbilical cord mesenchymal stem cells (HUC-MSCs) 
      are promising candidates for cell-based therapy in regenerative medicine or other 
      diseases due to their superior characteristics, including higher proliferation, 
      faster self-renewal ability, lower immunogenicity, a noninvasive harvest 
      procedure, easy expansion in vitro, and ethical access, compared with stem cells 
      from other sources. METHODS AND RESULTS: In the present study, we knocked down 
      the expression of SOX9 in HUC-MSCs by lentivirus interference and found that 
      knockdown of SOX9 inhibited the proliferation and migration of HUC-MSCs and 
      influenced the expression of cytokines (IL-6 and IL-8), growth factors (GM-CSF 
      and VEGF) and stemness-related genes (OCT4 and SALL4). In addition, the repair 
      effect of skin with burn injury in rats treated with HUC-MSCs transfected with 
      sh-control was better than that rats treated with HUC-MSCs transfected with 
      shSOX9 or PBS, and the accessory structures of the skin, including hair follicles 
      and glands, were greater than those in the other groups. We found that knockdown 
      of the expression of SOX9 obviously inhibited the expression of Ki67, CK14 and 
      CK18. CONCLUSIONS: In conclusion, this study will provide a guide for modifying 
      HUC-MSCs by bioengineering technology in the future.
FAU - Bie, Qingli
AU  - Bie Q
AD  - Department of Laboratory Medicine, Affiliated Hospital of Jining Medical 
      University, Jining Medical University, Jining, China.
AD  - Institute of Forensic Medicine and Laboratory Medicine, Jining Medical 
      University, Jining, China.
FAU - Zhai, Ruixia
AU  - Zhai R
AD  - Department of Obstetric, Affiliated Hospital of Jining Medical University, Jining 
      Medical University, Jining, China.
FAU - Chen, Yanrong
AU  - Chen Y
AD  - Department of Laboratory Medicine, Affiliated Hospital of Jining Medical 
      University, Jining Medical University, Jining, China.
FAU - Li, Yingao
AU  - Li Y
AD  - Department of Laboratory Medicine, Affiliated Hospital of Jining Medical 
      University, Jining Medical University, Jining, China.
FAU - Xie, Na
AU  - Xie N
AD  - Department of Laboratory Medicine, Affiliated Hospital of Jining Medical 
      University, Jining Medical University, Jining, China.
FAU - Wang, Baoyi
AU  - Wang B
AD  - Institute of Forensic Medicine and Laboratory Medicine, Jining Medical 
      University, Jining, China.
FAU - Yuan, Poyun
AU  - Yuan P
AD  - Institute of Forensic Medicine and Laboratory Medicine, Jining Medical 
      University, Jining, China.
FAU - Zhou, Xinjie
AU  - Zhou X
AD  - Institute of Forensic Medicine and Laboratory Medicine, Jining Medical 
      University, Jining, China.
FAU - Cong, Haiyan
AU  - Cong H
AD  - Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of 
      Medicine, Weihai, China.
FAU - Chang, Xin
AU  - Chang X
AD  - Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of 
      Medicine, Weihai, China.
FAU - Xiong, Huabao
AU  - Xiong H
AD  - Institute of Immunology and Molecular Medicine, Jining Medical University, 
      Jining, China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - Department of Laboratory Medicine, Affiliated Hospital of Jining Medical 
      University, Jining Medical University, Jining, China.
AD  - Institute of Forensic Medicine and Laboratory Medicine, Jining Medical 
      University, Jining, China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Int J Stem Cells
JT  - International journal of stem cells
JID - 101497587
PMC - PMC8611311
OTO - NOTNLM
OT  - HUC-MSCs
OT  - Regenerative medicine
OT  - SOX9
OT  - Skin
COIS- Potential Conflict of Interest The authors have no conflicting financial 
      interest.
EDAT- 2021/08/31 06:00
MHDA- 2021/08/31 06:01
PMCR- 2021/08/31
CRDT- 2021/08/30 05:37
PHST- 2021/04/20 00:00 [received]
PHST- 2021/06/09 00:00 [revised]
PHST- 2021/07/08 00:00 [accepted]
PHST- 2021/08/31 06:00 [pubmed]
PHST- 2021/08/31 06:01 [medline]
PHST- 2021/08/30 05:37 [entrez]
PHST- 2021/08/31 00:00 [pmc-release]
AID - ijsc21078 [pii]
AID - ijsc-14-4-465 [pii]
AID - 10.15283/ijsc21078 [doi]
PST - ppublish
SO  - Int J Stem Cells. 2021 Nov 30;14(4):465-474. doi: 10.15283/ijsc21078.