PMID- 34457020
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210831
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2021
DP  - 2021
TI  - Inhibitory Effect of Gualou Guizhi Decoction on Microglial Inflammation and 
      Neuron Injury by Promoting Anti-Inflammation via Targeting mmu-miR-155.
PG  - 2549076
LID - 10.1155/2021/2549076 [doi]
LID - 2549076
AB  - Gualou Guizhi decoction (GLGZD) treatment exerts neuroprotective effects and 
      promotes spasticity following ischemic stroke. However, the molecular mechanism 
      of GLGZD treatment on ischemic stroke remains unclear. Our previous study 
      indicated that GLGZD ameliorates neuronal damage caused by secondary inflammatory 
      injury induced by microglia. In the present study, we investigate the potential 
      mechanism of GLGZD treatment on neuron damage induced by neuroinflammation via 
      mmu-miR-155 in vitro. The HT22 cell line and the BV2 cell line were exposed to 
      oxygen/glucose-deprive (OGD) conditions; the conditioned medium was prepared 
      using the supernatants from OGD-stimulated BV2 cells after pretreating with 
      GLGZD. Cell viability was determined by MTT assays; levels of released 
      inflammatory cytokines were assessed using the BioPlex system. mmu-miR-155 and 
      its targeting genes were detected using real-time reverse transcription 
      polymerase chain reaction (RT-PCR). The expression of anti-inflammatory proteins 
      was evaluated by Western blotting. DAPI staining was used to test the apoptotic 
      cells. Our results showed that GLGZD pretreatment significantly induced IL10 
      release and decreased the production of TNF-alpha, IL6, and IFN-gamma. In addition, GLGZD 
      markedly attenuated mmu-miR-155 expression and its downstream SOCS1, SMAD2, 
      SHIP1, and TAB2 expression levels. The DAPI-stained apoptotic cell death and 
      caspase-3 activation in HT22 cells exposed to the conditioned medium were 
      reversed by GLGZD treatment. Our findings suggested that GLGZD pretreatment 
      downregulates the mmu-miR-155 signaling, which inhibits microglial inflammation, 
      thereby resulting in the suppression of neuron apoptosis after OGD stress. The 
      underlying mechanisms may provide the support for GLGZD treatment of cerebral 
      ischemic injury.
CI  - Copyright (c) 2021 Haixia Hu et al.
FAU - Hu, Haixia
AU  - Hu H
AUID- ORCID: 0000-0003-1308-5739
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, China.
AD  - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, China.
FAU - Zhong, Xinghua
AU  - Zhong X
AUID- ORCID: 0000-0001-6366-227X
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, China.
AD  - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, China.
FAU - Lin, Xinjun
AU  - Lin X
AUID- ORCID: 0000-0002-2757-0535
AD  - Institute of Integrated Chinese and Western Medicine, Fujian University of 
      Traditional Chinese Medicine, Fuzhou, China.
FAU - Yang, Jinbo
AU  - Yang J
AUID- ORCID: 0000-0002-7467-5188
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, China.
AD  - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, China.
FAU - Zhu, Xiaoqin
AU  - Zhu X
AUID- ORCID: 0000-0002-8250-7176
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, China.
AD  - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210817
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC8387184
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2021/08/31 06:00
MHDA- 2021/08/31 06:01
PMCR- 2021/08/17
CRDT- 2021/08/30 05:51
PHST- 2021/04/11 00:00 [received]
PHST- 2021/07/02 00:00 [revised]
PHST- 2021/08/11 00:00 [accepted]
PHST- 2021/08/30 05:51 [entrez]
PHST- 2021/08/31 06:00 [pubmed]
PHST- 2021/08/31 06:01 [medline]
PHST- 2021/08/17 00:00 [pmc-release]
AID - 10.1155/2021/2549076 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2021 Aug 17;2021:2549076. doi: 
      10.1155/2021/2549076. eCollection 2021.