PMID- 34462500
OWN - NLM
STAT- MEDLINE
DCOM- 20211109
LR  - 20211109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Aug 30
TI  - A genetic screen for Drosophila social isolation mutants and analysis of sex 
      pistol.
PG  - 17395
LID - 10.1038/s41598-021-96871-x [doi]
LID - 17395
AB  - Prolonged periods of forced social isolation is detrimental to well-being, yet we 
      know little about which genes regulate susceptibility to its effects. In the 
      fruit fly, Drosophila melanogaster, social isolation induces stark changes in 
      behavior including increased aggression, locomotor activity, and resistance to 
      ethanol sedation. To identify genes regulating sensitivity to isolation, I 
      screened a collection of sixteen hundred P-element insertion lines for mutants 
      with abnormal levels of all three isolation-induced behaviors. The screen 
      identified three mutants whose affected genes are likely central to regulating 
      the effects of isolation in flies. One mutant, sex pistol (sxp), became extremely 
      aggressive and resistant to ethanol sedation when socially isolated. sxp also had 
      a high level of male-male courtship. The mutation in sxp reduced the expression 
      of two minor isoforms of the actin regulator hts (adducin), as well as mildly 
      reducing expression of CalpA, a calcium-dependent protease. As a consequence, sxp 
      also had increased expression of the insulin-like peptide, dILP5. Analysis of the 
      social behavior of sxp suggests that these minor hts isoforms function to limit 
      isolation-induced aggression, while chronically high levels of dILP5 increase 
      male-male courtship.
CI  - (c) 2021. The Author(s).
FAU - Eddison, Mark
AU  - Eddison M
AD  - Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, 
      Ashburn, VA, 20147, USA. eddisonm@janelia.hhmi.org.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210830
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Calmodulin-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Ilp5 protein, Drosophila)
RN  - 0 (Insulins)
RN  - 0 (Protein Isoforms)
RN  - 0 (adducin)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (calpain A, Drosophila)
SB  - IM
MH  - Aggression
MH  - Animals
MH  - Calmodulin-Binding Proteins/genetics/metabolism
MH  - Calpain/genetics/metabolism
MH  - Courtship
MH  - Down-Regulation
MH  - Drosophila/genetics/*physiology
MH  - Drosophila Proteins/*genetics/metabolism
MH  - Insulins/genetics/metabolism
MH  - Locomotion
MH  - Male
MH  - Mutation
MH  - Protein Isoforms/genetics/metabolism
MH  - *Social Isolation
MH  - Up-Regulation
PMC - PMC8405609
COIS- The author declares no competing interests.
EDAT- 2021/09/01 06:00
MHDA- 2021/11/10 06:00
PMCR- 2021/08/30
CRDT- 2021/08/31 06:22
PHST- 2021/01/31 00:00 [received]
PHST- 2021/08/10 00:00 [accepted]
PHST- 2021/08/31 06:22 [entrez]
PHST- 2021/09/01 06:00 [pubmed]
PHST- 2021/11/10 06:00 [medline]
PHST- 2021/08/30 00:00 [pmc-release]
AID - 10.1038/s41598-021-96871-x [pii]
AID - 96871 [pii]
AID - 10.1038/s41598-021-96871-x [doi]
PST - epublish
SO  - Sci Rep. 2021 Aug 30;11(1):17395. doi: 10.1038/s41598-021-96871-x.