PMID- 34462642
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210901
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2021
DP  - 2021
TI  - The RIG-I Signal Pathway Mediated Panax notoginseng Saponin Anti-Inflammatory 
      Effect in Ischemia Stroke.
PG  - 8878428
LID - 10.1155/2021/8878428 [doi]
LID - 8878428
AB  - Panax notoginseng saponins (PNS), the main bioactive constituents of a 
      traditional Chinese herb Panax notoginseng, were commonly used for ischemic 
      stroke in China. However, the associated cellular and molecular mechanisms of PNS 
      have not been well examined. This study aimed to decipher the underlying 
      molecular target of PNS in the treatment of cerebral ischemia. The 
      oxygen-glucose-deprived (OGD) model of rat brain microvascular endothelial cells 
      (BMECs) was used in this study. The alteration of gene expression in rat BMECs 
      after PNS treatment was measured by microarray and indicated that there were 38 
      signaling pathways regulated by PNS. Among them, RIG-I receptor and related 
      signaling molecules TNF receptor-associated factor 2 (Traf2) and nuclear 
      factor-kappa B (NF-kappaB) were significantly suppressed by PNS, which was verified 
      again in OGD-induced BMECs measured by FQ-PCR and western blotting and in middle 
      cerebral artery occlusion (MCAO) rats measured by immunohistochemistry. The 
      levels of TNF-alpha, IL-8, and the downstream cytokines regulated by RIG-I receptor 
      pathway were also decreased by PNS. Meanwhile, the neurological evaluation, 
      hematoxylin and eosin (HE) staining, and Evans blue staining were conducted to 
      evaluate the effect of PNS in MCAO rats. Results showed PNS significantly 
      improved functional outcome and cerebral vascular leakage. Flow cytometry showed 
      the number of the inflammatory cells infiltrated in brain tissue was decreased in 
      PNS treatment. Our results identified that RIG-I signaling pathway mediated 
      anti-inflammatory properties of PNS in cerebral ischemia, which provided the 
      novel insights of PNS application in clinics.
CI  - Copyright (c) 2021 Chujun Zhang et al.
FAU - Zhang, Chujun
AU  - Zhang C
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
AD  - Nanchang Hongdu Hospital of Traditional Chinese Medicine, Nanchang, China.
FAU - Zhang, Sai
AU  - Zhang S
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Wang, Lanxiang
AU  - Wang L
AD  - Department of Traditional Chinese Medicine, The 8th Medical Center of Chinese PLA 
      General Hospital, Beijing, China.
FAU - Kang, Soyeon
AU  - Kang S
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Ma, Jiabao
AU  - Ma J
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Liu, Shihao
AU  - Liu S
AD  - Department of Cell and Developmental Biology, School of Molecular and Cellular 
      Biology, University of Illinois at Urbana-Champaign, USA.
FAU - Hu, Yanhong
AU  - Hu Y
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Sun, Tianshi
AU  - Sun T
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Dong, Yixin
AU  - Dong Y
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Li, Tenghui
AU  - Li T
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Li, Weihong
AU  - Li W
AUID- ORCID: 0000-0002-8047-8201
AD  - School of Nursing, Beijing University of Chinese Medicine, Beijing, China.
FAU - Du, Qinghong
AU  - Du Q
AUID- ORCID: 0000-0002-3753-5995
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, China.
AD  - Institute of Tibetan Medicine, University of Tibetan Medicine, Lhasa, China.
LA  - eng
PT  - Journal Article
DEP - 20210820
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC8403041
COIS- The authors declare no conflicts of interest.
EDAT- 2021/09/01 06:00
MHDA- 2021/09/01 06:01
PMCR- 2021/08/20
CRDT- 2021/08/31 06:39
PHST- 2020/09/07 00:00 [received]
PHST- 2021/02/09 00:00 [revised]
PHST- 2021/08/07 00:00 [accepted]
PHST- 2021/08/31 06:39 [entrez]
PHST- 2021/09/01 06:00 [pubmed]
PHST- 2021/09/01 06:01 [medline]
PHST- 2021/08/20 00:00 [pmc-release]
AID - 10.1155/2021/8878428 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2021 Aug 20;2021:8878428. doi: 
      10.1155/2021/8878428. eCollection 2021.