PMID- 34465532
OWN - NLM
STAT- MEDLINE
DCOM- 20220318
LR  - 20220318
IS  - 1440-1592 (Electronic)
IS  - 1323-8930 (Linking)
VI  - 71
IP  - 1
DP  - 2022 Jan
TI  - A novel detection method for cross-linking of IgE-receptors by autoantibodies in 
      chronic spontaneous urticaria.
PG  - 94-102
LID - S1323-8930(21)00095-2 [pii]
LID - 10.1016/j.alit.2021.08.007 [doi]
AB  - BACKGROUND: Autoantibodies (AAbs) against immunoglobulin E (IgE) antibodies (Abs) 
      and their high-affinity receptor alpha subunits (FcepsilonRIalpha) are key factors in the 
      elicitation of type IIb autoimmune chronic spontaneous urticaria (type IIb 
      aiCSU). In this study, we aimed to develop a new method to detect functional 
      anti-FcepsilonRIalpha and anti-IgE AAbs, which can crosslink the plural FcepsilonRcapital I, Ukrainianalpha molecules 
      and IgE Abs on the surface of mast cells and basophils, in sera from aiCSU 
      patients using the amplified luminescence proximity homogeneous assay (Alpha). 
      METHODS: Sera were obtained from 14 aiCSU patients, as diagnosed by recurrent 
      chronic spontaneous urticaria episodes and positive results for the autologous 
      serum skin test and/or histamine release test (HRT). The AAbs to FcepsilonRIalpha and IgE 
      Abs were determined in sera from aiCSU patients using enzyme-linked immunosorbent 
      assay (ELISA) and Alpha by cross-linking (AlphaCL) of IgE Abs and/or FcepsilonRcapital I, Ukrainianalpha. 
      RESULTS: Serum anti-FcepsilonRIalpha and anti-IgE AAb levels were not significantly 
      different between aiCSU patients and healthy subjects in ELISA. Anti-FcepsilonRIalpha AAbs 
      were detected in 10 of 14 aiCSU patients who displayed positive (5/5) and 
      negative (5/9) results in the HRT for anti-FcepsilonRIalpha AAbs by AlphaCL, whereas no 
      signals were observed in healthy subjects. Additionally, anti-IgE AAbs were 
      detected in two of four aiCSU patients who displayed positive results in the HRT 
      for anti-IgE AAbs. CONCLUSIONS: A new assay method using AlphaCL can detect 
      anti-FcepsilonRIalpha and anti-IgE AAbs with FcepsilonRIalpha- and IgE-crosslinking abilities in sera 
      from aiCSU patients. This simple and practical assay method may be available as a 
      diagnostic tool for urticaria patients.
CI  - Copyright (c) 2021 Japanese Society of Allergology. Production and hosting by 
      Elsevier B.V. All rights reserved.
FAU - Koga, Yuki
AU  - Koga Y
AD  - Department of Pharmaceutical Services, Graduate School of Biomedical and Health 
      Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Yokooji, Tomoharu
AU  - Yokooji T
AD  - Department of Pharmaceutical Services, Graduate School of Biomedical and Health 
      Sciences, Hiroshima University, Hiroshima, Japan; Department of Frontier Science 
      for Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima 
      University, Hiroshima, Japan.
FAU - Ogino, Ryohei
AU  - Ogino R
AD  - Department of Frontier Science for Pharmacotherapy, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Taogoshi, Takanori
AU  - Taogoshi T
AD  - Department of Pharmaceutical Services, Graduate School of Biomedical and Health 
      Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Takahagi, Shunsuke
AU  - Takahagi S
AD  - Department of Dermatology, Graduate School of Biomedical and Health Sciences, 
      Hiroshima University, Hiroshima, Japan.
FAU - Ishii, Kaori
AU  - Ishii K
AD  - Department of Dermatology, Graduate School of Biomedical and Health Sciences, 
      Hiroshima University, Hiroshima, Japan.
FAU - Chinuki, Yuko
AU  - Chinuki Y
AD  - Department of Dermatology, Shimane University Faculty of Medicine, Izumo, Japan.
FAU - Morita, Eishin
AU  - Morita E
AD  - Department of Dermatology, Shimane University Faculty of Medicine, Izumo, Japan.
FAU - Hide, Michihiro
AU  - Hide M
AD  - Department of Dermatology, Graduate School of Biomedical and Health Sciences, 
      Hiroshima University, Hiroshima, Japan.
FAU - Matsuo, Hiroaki
AU  - Matsuo H
AD  - Department of Pharmaceutical Services, Graduate School of Biomedical and Health 
      Sciences, Hiroshima University, Hiroshima, Japan. Electronic address: 
      hmatsuo@hiroshima-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20210828
PL  - England
TA  - Allergol Int
JT  - Allergology international : official journal of the Japanese Society of 
      Allergology
JID - 9616296
RN  - 0 (Autoantibodies)
RN  - 0 (FCER1A protein, human)
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Autoantibodies/*immunology
MH  - Case-Control Studies
MH  - Chronic Urticaria/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Histamine Release
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Receptors, IgE/antagonists & inhibitors/blood/*immunology
MH  - Skin/chemistry
MH  - Skin Tests
OTO - NOTNLM
OT  - Amplified luminescence proximity homogeneous assay
OT  - Autoantibody
OT  - Chronic spontaneous urticaria
OT  - Cross-linking
OT  - FcepsilonRIalpha
EDAT- 2021/09/02 06:00
MHDA- 2022/03/19 06:00
CRDT- 2021/09/01 05:53
PHST- 2021/06/21 00:00 [received]
PHST- 2021/07/14 00:00 [revised]
PHST- 2021/07/24 00:00 [accepted]
PHST- 2021/09/02 06:00 [pubmed]
PHST- 2022/03/19 06:00 [medline]
PHST- 2021/09/01 05:53 [entrez]
AID - S1323-8930(21)00095-2 [pii]
AID - 10.1016/j.alit.2021.08.007 [doi]
PST - ppublish
SO  - Allergol Int. 2022 Jan;71(1):94-102. doi: 10.1016/j.alit.2021.08.007. Epub 2021 
      Aug 28.