PMID- 34466285
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210903
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 9
DP  - 2021
TI  - The whole-genome and expression profile analysis of WRKY and RGAs in Dactylis 
      glomerata showed that DG6C02319.1 and DgWRKYs may cooperate in the immunity 
      against rust.
PG  - e11919
LID - 10.7717/peerj.11919 [doi]
LID - e11919
AB  - Orchardgrass (Dactylis glomerata) is one of the top four perennial forages 
      worldwide and, despite its large economic advantages, often threatened by various 
      environmental stresses. WRKY transcription factors (TFs) can regulate a variety 
      of plant processes, widely participate in plant responses to biotic and abiotic 
      stresses, and are one of the largest gene families in plants. WRKYs can usually 
      bind W-box elements specifically. In this study, we identified a total of 93 
      DgWRKY genes and 281 RGAs, including 65, 169 and 47 nucleotide-binding 
      site-leucine-rich repeats (NBS-LRRs), leucine-rich repeats receptor-like protein 
      kinases (LRR-RLKs), and leucine-rich repeats receptor-like proteins (LRR-RLPs), 
      respectively. Through analyzing the expression of DgWRKY genes in orchardgrass 
      under different environmental stresses, it was found that many DgWRKY genes were 
      differentially expressed under heat, drought, submergence, and rust stress. In 
      particular, it was found that the greatest number of genes were differentially 
      expressed under rust infection. Consistently, GO and KEGG enrichment analysis of 
      all genes showed that 78 DgWRKY TFs were identified in the plant-pathogen 
      interaction pathway, with 59 of them differentially expressed. Through cis-acting 
      element prediction, 154 RGAs were found to contain W-box elements. Among them, 
      DG6C02319.1 (a member of the LRR-RLK family) was identified as likely to interact 
      with 14 DGWRKYs. Moreover, their expression levels in susceptible plants after 
      rust inoculation were first up-regulated and then down-regulated, while those in 
      the resistant plants were always up-regulated. In general, DgWRKYs responded to 
      both biotic stress and abiotic stress. DgWRKYs and RGAs may synergistically 
      respond to the response of orchardgrass to rust. This study provides meaningful 
      insight into the molecular mechanisms of WRKY proteins in orchardgrass.
CI  - (c) 2021 Ren et al.
FAU - Ren, Juncai
AU  - Ren J
AD  - College of Animal Science and Technology, Southwest University, Chongqing, 
      Chongqing, China.
FAU - Hu, Jialing
AU  - Hu J
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Zhang, Ailing
AU  - Zhang A
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Ren, Shuping
AU  - Ren S
AD  - College of Animal Science and Technology, Southwest University, Chongqing, 
      Chongqing, China.
FAU - Jing, Tingting
AU  - Jing T
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Wang, Xiaoshan
AU  - Wang X
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Sun, Min
AU  - Sun M
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Huang, Linkai
AU  - Huang L
AUID- ORCID: 0000-0001-7810-4852
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan, China.
FAU - Zeng, Bing
AU  - Zeng B
AD  - College of Animal Science and Technology, Southwest University, Chongqing, 
      Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20210819
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC8380429
OTO - NOTNLM
OT  - Abiotic stress
OT  - Dactylis glomerate
OT  - RGAs
OT  - Rust stress
OT  - Transcriptome
OT  - WRKY family
COIS- The authors declare that they have no competing interests.
EDAT- 2021/09/02 06:00
MHDA- 2021/09/02 06:01
PMCR- 2021/08/19
CRDT- 2021/09/01 07:24
PHST- 2021/02/15 00:00 [received]
PHST- 2021/07/16 00:00 [accepted]
PHST- 2021/09/01 07:24 [entrez]
PHST- 2021/09/02 06:00 [pubmed]
PHST- 2021/09/02 06:01 [medline]
PHST- 2021/08/19 00:00 [pmc-release]
AID - 11919 [pii]
AID - 10.7717/peerj.11919 [doi]
PST - epublish
SO  - PeerJ. 2021 Aug 19;9:e11919. doi: 10.7717/peerj.11919. eCollection 2021.