PMID- 34467691 OWN - NLM STAT- MEDLINE DCOM- 20210902 LR - 20210902 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 46 IP - 12 DP - 2021 Jun TI - [Potential targets of Euodiae Fructus in treatment of insomnia based on network pharmacology]. PG - 3016-3023 LID - 10.19540/j.cnki.cjcmm.20210419.401 [doi] AB - The acupoint application of Euodiae Fructus at Yongquan(KI1) can significantly improve the sleep quality of patients with insomnia with berberine as the main effective component for the efficacy. Nineteen active compounds and 203 drug targets were screened out from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). After comparison with GeneCards and Online Mendelian Inheritance in Man(OMIM), 24 common genes of diseases and drugs were obtained. STRING 11.0 was used to construct a protein-protein interaction(PPI) network of the overlapping genes, and Matthews correlation coefficient(MCC) was employed to screen the core genes, which were then subjected to enrichment analysis with gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG). The results revealed that the main compounds of Euodiae Fructus, such as berberine and rutaecarpine, participated in the biological processes(such as neurotransmitter receptor activity) by regulating C-reactive protein(CRP), estrogen receptor 1(ESR1), 5-hydroxytryptamine(5-HT) receptor, and interleukin-6(IL-6) to exert sedative, anxiolytic, and antidepressant effects. Sixty 4-week-old SPF mice were randomly divided into a control group, a model group, a positive drug(diazepam tablets) group, and low-, medium-, and high-dose berberine groups. Medication with corresponding drugs was performed for one week. The results demonstrated that berberine was potent in reducing the activities and standing times of mice, down-regulating the levels of CRP and IL-6 mRNA in the hypothalamus, and up-regulating the expression of 5-HT(P<0.01); however, no significant effect on ESR1 was observed. The network of Euodiae Fructus in treating insomnia was constructed by network pharmacology and verified by tests. The findings indicated that the therapeutic efficacy of Euodiae Fructus in treating insomnia was achieved by participating in multiple biological processes, such as neurotransmitter receptor activity, which provided a scientific basis for its clinical application. FAU - Li, Lei AU - Li L AD - the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Lu, Jing-Jing AU - Lu JJ AD - the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Wang, Wei-Tao AU - Wang WT AD - Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Wang, Na AU - Wang N AD - the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Wang, Li-Xiang AU - Wang LX AD - Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Ma, Yan-Min AU - Ma YM AD - Henan University of Chinese Medicine Zhengzhou 450000, China. FAU - Liu, Shu AU - Liu S AD - the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China. LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Drugs, Chinese Herbal) SB - IM MH - Animals MH - Databases, Genetic MH - *Drugs, Chinese Herbal/pharmacology MH - Gene Ontology MH - Humans MH - Medicine, Chinese Traditional MH - Mice MH - *Sleep Initiation and Maintenance Disorders/drug therapy/genetics OTO - NOTNLM OT - Euodiae Fructus OT - berberine OT - insomnia OT - network pharmacology EDAT- 2021/09/02 06:00 MHDA- 2021/09/03 06:00 CRDT- 2021/09/01 07:53 PHST- 2021/09/01 07:53 [entrez] PHST- 2021/09/02 06:00 [pubmed] PHST- 2021/09/03 06:00 [medline] AID - 10.19540/j.cnki.cjcmm.20210419.401 [doi] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2021 Jun;46(12):3016-3023. doi: 10.19540/j.cnki.cjcmm.20210419.401.