PMID- 34468120
OWN - NLM
STAT- MEDLINE
DCOM- 20210920
LR  - 20220716
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 7
IP  - 9
DP  - 2021 Sep 13
TI  - Remote-Controlled 3D Porous Magnetic Interface toward High-Throughput Dynamic 3D 
      Cell Culture.
PG  - 4535-4544
LID - 10.1021/acsbiomaterials.1c00459 [doi]
AB  - Mechanical stimuli have been shown to play a large role in cellular behavior, 
      including cellular growth, differentiation, morphology, homeostasis, and disease. 
      Therefore, developing bioreactor systems that can create complex mechanical 
      environments for both tissue engineering and disease modeling drug screening is 
      appealing. However, many of existing systems are restricted because of their 
      bulky size with external force generators, destructive microenvironment control, 
      and low throughput. These shortcomings have preceded to the utilization of 
      magnetic stimuli responsive materials, given their untethered, fast, and tunable 
      actuation potential at both the microscale and macroscale level, for seamless 
      integration into cell culture wells and microfluidic systems. Nevertheless, 
      magnetic soft materials for cell culture have been limited due to the inability 
      to develop well-defined 3D structures for more complex and physiological relevant 
      mechanical actuation. Herein, we introduce a facile fabrication process to 
      develop magnetic-PDMS (polydimethylsiloxane) porous composite designs with both 
      well-defined and controllable microlevel and macrolevel features to dynamically 
      manipulate 3D cell-laden gel at the scale. The intrinsic stiffness of the 
      magnetic-PDMS porous composites is also modulated to control the deformation 
      potential to mimic physiological relevant strain levels, with 2.89-11% observed 
      in magnetic actuation studies. High cell viability was achieved with the 
      culturing of both human adipose stem cells (hADMSCs) and human umbilical cord 
      mesenchymal stem cells (hUCMSCs) in 3D cell-laden gel interfaced with the 
      magnetic-PDMS porous composite. Also, the highly interconnected porous network of 
      the magnetic-PDMS composites facilitated free diffusion throughout the porous 
      structure showcasing the potential of a multisurface contact 3D porous magnetic 
      structure in both reservoir and 96-well plate insert designs for more complex 
      dynamic mechanical actuation. In conclusion, these studies provide a means for 
      establishing a biocompatible, tunable magnetic-PDMS porous composite with fast 
      and programmable dynamic strain potential making it a suitable platform for 
      high-throughput, dynamic 3D cell culture.
FAU - Stottlemire, Bryce J
AU  - Stottlemire BJ
AD  - Department of Chemical and Petroleum Engineering, Bioengineering Program, 
      University of Kansas, Lawrence, Kansas 66045, United States.
FAU - Chakravarti, Aparna R
AU  - Chakravarti AR
AD  - Department of Chemical and Petroleum Engineering, Bioengineering Program, 
      University of Kansas, Lawrence, Kansas 66045, United States.
FAU - Whitlow, Jonathan W
AU  - Whitlow JW
AD  - Department of Chemical and Petroleum Engineering, Bioengineering Program, 
      University of Kansas, Lawrence, Kansas 66045, United States.
FAU - Berkland, Cory J
AU  - Berkland CJ
AUID- ORCID: 0000-0002-9346-938X
AD  - Department of Chemical and Petroleum Engineering, Bioengineering Program, 
      University of Kansas, Lawrence, Kansas 66045, United States.
AD  - Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 
      66045, United States.
FAU - He, Mei
AU  - He M
AUID- ORCID: 0000-0001-5316-9326
AD  - Department of Chemical and Petroleum Engineering, Bioengineering Program, 
      University of Kansas, Lawrence, Kansas 66045, United States.
AD  - Department of Pharmaceutics, University of Florida, Gainesville, Florida 32608, 
      United States.
LA  - eng
GR  - R35 GM133794/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210901
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
SB  - IM
MH  - *Cell Culture Techniques
MH  - Cell Survival
MH  - Humans
MH  - Magnetic Phenomena
MH  - Porosity
MH  - *Tissue Engineering
PMC - PMC9217666
MID - NIHMS1809122
OTO - NOTNLM
OT  - 3D dynamic cell culture
OT  - high-throughput
OT  - magnetic responsive materials
OT  - remote actuation
OT  - stem cells
OT  - tissue engineering
EDAT- 2021/09/02 06:00
MHDA- 2021/09/21 06:00
PMCR- 2022/06/23
CRDT- 2021/09/01 12:14
PHST- 2021/09/02 06:00 [pubmed]
PHST- 2021/09/21 06:00 [medline]
PHST- 2021/09/01 12:14 [entrez]
PHST- 2022/06/23 00:00 [pmc-release]
AID - 10.1021/acsbiomaterials.1c00459 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2021 Sep 13;7(9):4535-4544. doi: 
      10.1021/acsbiomaterials.1c00459. Epub 2021 Sep 1.