PMID- 34468207
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20240226
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Linking)
VI  - 321
IP  - 5
DP  - 2021 Nov 1
TI  - LncRNA RGMB-AS1 facilitates pancreatic cancer cell proliferation and migration 
      but inhibits cell apoptosis via miR-574-3p/PIM3 axis.
PG  - G477-G488
LID - 10.1152/ajpgi.00443.2020 [doi]
AB  - Pancreatic cancer (PC) is among the most notorious malignancies worldwide. Long 
      noncoding RNA (lncRNA) repulsive guidance molecule bone morphogenetic protein 
      (BMP) coreceptor b antisense RNA 1 (RGMB-AS1) was an oncogene in glioma. However, 
      the RGMB-AS1 function in PC remains largely unknown. Herein, quantitative 
      real-time polymerase chain reaction was performed to analyze the expression of 
      RGMB-AS1. We determined RGMB-AS1 influence on PC cell malignant behaviors via 
      functional assays. Besides, we applied subcellular fractionation and fluorescence 
      in situ hybridization (FISH) assays to confirm the cellular distribution of 
      RGMB-AS1 in PC cells. We used mechanism assays to detect the regulatory axis of 
      RGMB-AS1 in PC cells. Briefly, the level of RGMB-AS1 expression in PC cells was 
      abnormally high. RGMB-AS1 knockdown impeded PC cell proliferation and migration, 
      but induced cell apoptosis, and RGMB-AS1 overexpression led the opposite 
      consequences. RGMB-AS1 acted as a competing endogenous RNA (ceRNA) to sequester 
      miR-574-3p and thereby regulated Pim-3 proto-oncogene, serine/threonine kinase 
      (PIM3) expression. Conclusively, our work revealed the cancer-promoting function 
      of RGMB-AS1 in PC and that the regulatory mechanism of the 
      RGMB-AS1/miR-574-3p/PIM3 axis might contribute to novel biomarker development in 
      PC treatment.NEW & NOTEWORTHY RGMB-AS1 promotes PC cell proliferation, elevates 
      PC cell migration capacity, inhibits PC cell apoptosis, and promotes PC cell 
      proliferation and migration but inhibits cell apoptosis via targeting miR-574-3p. 
      PIM3 is directly targeted by miR-574-3p.
FAU - Song, Wenchong
AU  - Song W
AD  - Gastroenterology Division, Weihai Municipal Hospital, Weihai, China.
FAU - Shi, Chengjian
AU  - Shi C
AUID- ORCID: 0000-0003-1489-2022
AD  - Department of Biliary Pancreatic Surgery, Tongji Hospital of Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20210901
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (MAS1 protein, human)
RN  - 0 (MIRN574 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long noncoding RNA RGMB-AS1, human)
RN  - EC 2.7.11.1 (PIM3 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Pancreatic Neoplasms/*enzymology/genetics/pathology
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Signal Transduction
MH  - Tumor Burden
MH  - Mice
OTO - NOTNLM
OT  - PIM3
OT  - RGMB-AS1
OT  - miR-574-3p
OT  - pancreatic cancer
EDAT- 2021/09/02 06:00
MHDA- 2021/11/09 06:00
CRDT- 2021/09/01 12:17
PHST- 2021/09/02 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/09/01 12:17 [entrez]
AID - 10.1152/ajpgi.00443.2020 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2021 Nov 1;321(5):G477-G488. doi: 
      10.1152/ajpgi.00443.2020. Epub 2021 Sep 1.