PMID- 34475979
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210904
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 4
DP  - 2021 Oct
TI  - Rosuvastatin protects PC12 cells from hypoxia/reoxygenation-induced injury by 
      inhibiting endoplasmic reticulum stress-induced apoptosis.
PG  - 1189
LID - 10.3892/etm.2021.10623 [doi]
LID - 1189
AB  - The endoplasmic reticulum stress (ERS) response serves an important role in 
      cerebral ischemia-reperfusion injury (CIRI). However, to the best of the our 
      knowledge, the effect of rosuvastatin on the ERS response in CIRI has not yet 
      been studied. In the present study, the effect of rosuvastatin on cell damage in 
      CIRI was investigated; furthermore, the effect of rosuvastatin on the ERS 
      response was explored. Firstly, a hypoxia/reoxygenation (H/R)-induced cell damage 
      model was established in PC12 cells. Cell viability was subsequently detected by 
      a Cell Counting Kit-8 assay. A lactate dehydrogenase kit was used to detect 
      cytotoxicity. TUNEL assay was then used to measure the extent of cell apoptosis, 
      and western blotting was used to analyze the expression levels of the 
      apoptosis-associated proteins Bax, Bcl-2, cleaved caspase-3 and cleaved 
      caspase-9. In addition, western blotting was used to detect the expression levels 
      of ERS-associated proteins, including phosphorylated (p)-protein kinase R-like 
      endoplasmic reticulum kinase (PERK), p-eukaryotic initiation factor 2alpha and other 
      proteins. Treatment with rosuvastatin led to an increased activity of H/R-induced 
      PC12 cells and a decrease in their cytotoxicity. Rosuvastatin also led to an 
      inhibition in apoptosis and ERS in H/R-induced PC12 cells. After administration 
      of the ERS response activator thapsigargin (TG), TG was found to reverse the 
      protective effect of rosuvastatin on injury of H/R-induced PC12 cells. Taken 
      together, these findings have shown that rosuvastatin is able to protect PC12 
      cells from H/R-induced injury via inhibiting ERS-induced apoptosis, providing a 
      strong theoretical basis for the use of rosuvastatin in the clinical treatment of 
      CIRI.
CI  - Copyright: (c) Gao et al.
FAU - Gao, Yu
AU  - Gao Y
AD  - Department of Neurosurgery, First Affiliated Hospital of Hunan University of 
      Traditional Chinese Medicine, Changsha, Hunan 410007, P.R. China.
FAU - Li, Libo
AU  - Li L
AD  - Department of Neurosurgery, First Affiliated Hospital of Hunan University of 
      Traditional Chinese Medicine, Changsha, Hunan 410007, P.R. China.
FAU - Yu, Jianbai
AU  - Yu J
AD  - Department of Neurosurgery, First Affiliated Hospital of Hunan University of 
      Traditional Chinese Medicine, Changsha, Hunan 410007, P.R. China.
FAU - Zhang, Zhanwei
AU  - Zhang Z
AD  - Department of Neurosurgery, First Affiliated Hospital of Hunan University of 
      Traditional Chinese Medicine, Changsha, Hunan 410007, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210817
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8406900
OTO - NOTNLM
OT  - PC12 cells
OT  - apoptosis
OT  - cerebral ischemia-reperfusion injury
OT  - endoplasmic reticulum stress
OT  - rosuvastatin
COIS- The authors declare that they have no competing interests.
EDAT- 2021/09/04 06:00
MHDA- 2021/09/04 06:01
PMCR- 2021/08/17
CRDT- 2021/09/03 06:49
PHST- 2020/12/29 00:00 [received]
PHST- 2021/04/23 00:00 [accepted]
PHST- 2021/09/03 06:49 [entrez]
PHST- 2021/09/04 06:00 [pubmed]
PHST- 2021/09/04 06:01 [medline]
PHST- 2021/08/17 00:00 [pmc-release]
AID - ETM-0-0-10623 [pii]
AID - 10.3892/etm.2021.10623 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Oct;22(4):1189. doi: 10.3892/etm.2021.10623. Epub 2021 Aug 17.