PMID- 34476662
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220418
IS  - 2523-899X (Electronic)
IS  - 2096-5230 (Print)
IS  - 2523-899X (Linking)
VI  - 41
IP  - 5
DP  - 2021 Oct
TI  - Comparison of LAMP, GeneXpert, Mycobacterial Culture, Smear Microscopy, TSPOT.TB, 
      TBAg/PHA Ratio for Diagnosis of Pulmonary Tuberculosis.
PG  - 1023-1028
LID - 10.1007/s11596-021-2404-4 [doi]
AB  - OBJECTIVE: To investigate the application value of loop-mediated isothermal 
      amplification (LAMP), GeneXpert, mycobacterial culture, smear microscopy, 
      TSPOT.TB (TSPOT), ratio of TB-specific antigen to phytohemagglutinin (TBAg/PHA 
      ratio) in the detection of mycobacterium tuberculosis in the bronchoalveolar 
      lavage fluid. METHODS: A retrospective analysis was performed on the patients who 
      underwent bronchoscopy from December 2018 to November 2019 in Tongji Hospital. 
      The patients with positive tuberculosis culture or positive GeneXpert in 
      bronchoalveolar lavage fluid were selected as the case group, and those without 
      tuberculosis served as the control group. The receiver operating characteristic 
      (ROC) curve was used to evaluate the diagnostic value of LAMP, GeneXpert, 
      culture, smear microscopy, TSPOT, and TBAg/PHA ratio. RESULTS: For the patients 
      with positive cultures as case, the sensitivity of LAMP, GeneXpert, smear 
      microscopy, TSPOT and TBAg/PHA ratio was 73.49%, 89.16%, 25.30%, 80.00%, 33.85%, 
      respectively, the specificity was 99.00%, 100.00%, 99.00%, 86.00%, 100.00%, 
      respectively, the area under the ROC curve (AUC) was 0.849, 0.938, 0.633, 0.830, 
      0.669, respectively. For the patients with positive GeneXpert as case, the 
      sensitivity of LAMP, mycobacterial culture, smear microscopy, TSPOT and TBAg/PHA 
      ratio was 73.20%, 74.23%, 22.68%, 68.92%, 29.73%, respectively, the specificity 
      was 99.00%, 100.00%, 99.00%, 86.00%, 100.00%, respectively, the AUC was 0.853, 
      0.878, 0.623, 0.775, 0.649, respectively. CONCLUSION: The sensitivity of 
      GeneXpert was best. The sensitivity and diagnostic value of LAMP were slightly 
      lower than those of GeneXpert, and were similar to tuberculosis culture. The 
      sensitivity of smear microscopy was low. The specificity of TSPOT was low. When 
      TBAg/PHA ratio >0.2 was used as a diagnostic index, the specificity was improved, 
      but the sensitivity was low.
CI  - (c) 2021. Huazhong University of Science and Technology.
FAU - Deng, Yan
AU  - Deng Y
AD  - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, 430030, 
      China.
FAU - Duan, Yi-Fei
AU  - Duan YF
AD  - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, 430030, 
      China.
FAU - Gao, Shu-Pei
AU  - Gao SP
AD  - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, 430030, 
      China.
FAU - Wang, Jian-Miao
AU  - Wang JM
AD  - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, 430030, 
      China. wangjianmiao2010@126.com.
AD  - Department of Infection Control, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China. 
      wangjianmiao2010@126.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20210902
PL  - China
TA  - Curr Med Sci
JT  - Current medical science
JID - 101729993
RN  - LAMP assay
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bacteriological Techniques
MH  - Bronchoalveolar Lavage Fluid/*microbiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - Mycobacterium tuberculosis/genetics/growth & development/immunology/*isolation & 
      purification
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - ROC Curve
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Tuberculosis, Pulmonary/*diagnosis
PMC - PMC8412392
OTO - NOTNLM
OT  - GeneXpert
OT  - TSPOT
OT  - bronchoalveolar lavage fluid
OT  - loop-mediated isothermal amplification
OT  - pulmonary tuberculosis
EDAT- 2021/09/04 06:00
MHDA- 2022/02/05 06:00
PMCR- 2021/09/02
CRDT- 2021/09/03 07:04
PHST- 2020/11/26 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/09/04 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/09/03 07:04 [entrez]
PHST- 2021/09/02 00:00 [pmc-release]
AID - 10.1007/s11596-021-2404-4 [pii]
AID - 2404 [pii]
AID - 10.1007/s11596-021-2404-4 [doi]
PST - ppublish
SO  - Curr Med Sci. 2021 Oct;41(5):1023-1028. doi: 10.1007/s11596-021-2404-4. Epub 2021 
      Sep 2.