PMID- 34477472
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20231102
IS  - 1365-2060 (Electronic)
IS  - 0785-3890 (Print)
IS  - 0785-3890 (Linking)
VI  - 53
IP  - 1
DP  - 2021 Dec
TI  - LncRNA MEG8 sponging miR-181a-5p contributes to M1 macrophage polarization by 
      regulating SHP2 expression in Henoch-Schonlein purpura rats.
PG  - 1576-1588
LID - 10.1080/07853890.2021.1969033 [doi]
AB  - BACKGROUND: Long noncoding RNAs (LncRNAs) are regulatory molecules that play 
      important roles in various biological and pathological processes. Herein, we 
      aimed to explore whether maternally expressed gene 8 (MEG8) promotes M1 
      macrophage polarization among Henoch-Schonlein purpura (HSP) rats, and to 
      investigate the underlying mechanism. METHODS: Relative mRNA expression of MEG8, 
      miR-181a-5p and suppressor of SH2 domain-containing tyrosine phosphatase 2 (SHP2) 
      were examined using quantitative reverse transcription polymerase chain reaction. 
      Furthermore, expression of SHP2 and the Janus kinase 2/signal transducer and 
      activator of transcription 3 (JAK2/STAT3) pathway-related proteins was identified 
      using western blot. Luciferase activity assay was conducted to evaluate whether 
      miR-181a-5p could bind to MEG8 or SHP2. The macrophage phenotype was determined 
      using flow cytometry and enzyme-linked immunosorbent assay. RESULTS: We observed 
      macrophage polarization towards the M2 phenotype in the peripheral blood of HSP 
      rats. Furthermore, MEG8 and SHP2 expression were down-regulated, while 
      miR-181a-5p was up-regulated in monocyte-derived macrophages from the HSP rats 
      compared to the control group. Furthermore, MEG8 functioned as a sponge for 
      miR-181a-5p in order to facilitate SHP2 expression. Moreover, miR-181a-5p mimic 
      and SHP2 knockdown significantly reversed the MEG8 overexpression-mediated 
      suppression of JAK2/STAT3 signalling, and promotion of M1 polarization. 
      CONCLUSIONS: The lncRNA MEG8 sponged miR-181a-5p, which contributes to M1 
      macrophage polarization by regulating SHP2 expression in HSP rats.Key 
      MessagesLncRNA MEG8 downregulation and M2 polarization in Henoch Schonlein 
      purpura rats.MEG8 upregulation enhances M1 polarization and suppresses JAK2/STAT3 
      pathway.MEG8 sponges miRNA-181a-5p to regulate SHP2 expression.MiRNA-181a-5p 
      upregulation reverses lncRNA MEG8-mediated enhancement of M1 polarization and 
      inhibition of JAK2/STAT3 pathway.SHP2 downregulation reverses lncRNA 
      MEG8-mediated enhancement of M1 polarization and inhibition of JAK2/STAT3 
      pathway.
FAU - Jiang, Mingyu
AU  - Jiang M
AUID- ORCID: 0000-0002-0359-0064
AD  - Department of Pediatrics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
FAU - Dai, Jicheng
AU  - Dai J
AD  - Department of Pediatrics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
FAU - Yin, Mingying
AU  - Yin M
AD  - Department of Pediatrics, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
FAU - Jiang, Chunming
AU  - Jiang C
AD  - Department of Pediatrics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
FAU - Ren, Mingyong
AU  - Ren M
AUID- ORCID: 0000-0002-8779-2797
AD  - Department of Pediatrics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
FAU - Tian, Lin
AU  - Tian L
AD  - Department of Pathology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Med
JT  - Annals of medicine
JID - 8906388
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 2.7.10.2 (Janus Kinase 2)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Ptpn11 protein, rat)
SB  - IM
MH  - Animals
MH  - Down-Regulation
MH  - Humans
MH  - *IgA Vasculitis/genetics
MH  - Janus Kinase 2/genetics
MH  - Macrophages
MH  - MicroRNAs/*genetics/metabolism
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11/*genetics
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - STAT3 Transcription Factor/genetics
PMC - PMC8425717
OTO - NOTNLM
OT  - Henoch-Schonlein purpura
OT  - macrophage polarization
OT  - maternally expressed gene 8
OT  - miR-181a-5p
OT  - suppressor of SH2 domain-containing tyrosine phosphatase 2
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2021/09/04 06:00
MHDA- 2021/12/28 06:00
PMCR- 2021/09/03
CRDT- 2021/09/03 12:15
PHST- 2021/09/03 12:15 [entrez]
PHST- 2021/09/04 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2021/09/03 00:00 [pmc-release]
AID - 1969033 [pii]
AID - 10.1080/07853890.2021.1969033 [doi]
PST - ppublish
SO  - Ann Med. 2021 Dec;53(1):1576-1588. doi: 10.1080/07853890.2021.1969033.