PMID- 34483137
OWN - NLM
STAT- MEDLINE
DCOM- 20220119
LR  - 20220119
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 16
DP  - 2021 Aug 23
TI  - Hsa-circ-0007292 promotes the osteogenic differentiation of posterior 
      longitudinal ligament cells via regulating SATB2 by sponging miR-508-3p.
PG  - 20192-20217
LID - 10.18632/aging.203381 [doi]
AB  - Ossification of the posterior longitudinal ligament (OPLL) is a disorder with 
      multiple pathogenic mechanisms and leads to different degrees of neurological 
      symptoms. Recent studies have revealed that non-coding RNA (ncRNA), including 
      long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), could influence the 
      development of OPLL. Nevertheless, the molecular mechanisms linking circular RNAs 
      (circRNAs) and the progression of OPLL is still unknown. The current research 
      explored the expression profiles of OPLL-related circRNAs by microarray analysis, 
      and applied qRT-PCR to validate the results. Subsequently, we confirmed the 
      upregulation of hsa_circ_0007292 in OPLL cells by qRT-PCR and validated the 
      circular characteristic of hsa_circ_0007292 by Sanger sequencing. Fluorescence in 
      situ hybridization (FISH) unveiled that hsa_circ_0007292 was predominantly 
      located in the cytoplasm. Functionally, gain-of-function and loss-of-function 
      experiments showed that hsa_circ_0007292 promoted the osteogenic differentiation 
      of OPLL cells. Mechanistically, the interaction of hsa_circ_0007292 and 
      miR-508-3p was predicted and validated by bioinformatics analysis, 
      dual-luciferase reporter assays, and Ago2 RNA immunoprecipitation (RIP). 
      Similarly, we validated the correlation between miR-508-3p and SATB2. 
      Furthermore, rescue experiments were performed to prove that hsa_circ_0007292 
      acted as a sponge for miR-508-3p, and SATB2 was revealed to be the target gene of 
      miR-508-3p. In conclusion, our research shows that hsa_circ_0007292 regulates 
      OPLL progression by the miR-508-3p/SATB2 pathway. Our results indicate that 
      hsa_circ_0007292 can be used as a promising therapeutic target for patients with 
      OPLL.
FAU - Jiang, Anlong
AU  - Jiang A
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
AD  - The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of 
      Education, Harbin, China.
FAU - Wang, Nanxiang
AU  - Wang N
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Yan, Xinxing
AU  - Yan X
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Jiang, Yunheng
AU  - Jiang Y
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Song, Chengchao
AU  - Song C
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Chi, Hui
AU  - Chi H
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Chen, Guanghua
AU  - Chen G
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Wu, Feng
AU  - Wu F
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Ji, Ye
AU  - Ji Y
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Yan, Jinglong
AU  - Yan J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210823
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (MIRN508 microRNA, human)
RN  - 0 (Matrix Attachment Region Binding Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (SATB2 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Humans
MH  - Longitudinal Ligaments/cytology/metabolism
MH  - Matrix Attachment Region Binding Proteins/*genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Ossification of Posterior Longitudinal 
      Ligament/genetics/*metabolism/physiopathology
MH  - *Osteogenesis
MH  - RNA, Circular/genetics/*metabolism
MH  - Transcription Factors/*genetics/metabolism
MH  - Up-Regulation
PMC - PMC8436939
OTO - NOTNLM
OT  - ceRNA
OT  - circRNA
OT  - ossification
OT  - osteogenic differentiation
OT  - posterior longitudinal ligament
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2021/09/07 06:00
MHDA- 2022/01/20 06:00
PMCR- 2021/08/31
CRDT- 2021/09/06 05:40
PHST- 2021/04/26 00:00 [received]
PHST- 2021/07/15 00:00 [accepted]
PHST- 2021/09/06 05:40 [entrez]
PHST- 2021/09/07 06:00 [pubmed]
PHST- 2022/01/20 06:00 [medline]
PHST- 2021/08/31 00:00 [pmc-release]
AID - 203381 [pii]
AID - 10.18632/aging.203381 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2021 Aug 23;13(16):20192-20217. doi: 10.18632/aging.203381. 
      Epub 2021 Aug 23.