PMID- 34483913
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210907
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 12
DP  - 2021
TI  - Aspirin Attenuates Cardiac Allograft Rejection by Inhibiting the Maturation of 
      Dendritic Cells via the NF-kappaB Signaling Pathway.
PG  - 706748
LID - 10.3389/fphar.2021.706748 [doi]
LID - 706748
AB  - Background: Dendritic cells (DCs) serve as an important part of the immune system 
      and play a dual role in immune response. Mature DCs can initiate immune response, 
      while immature or semi-mature DCs induce immune hyporesponsiveness or tolerance. 
      Previous studies have shown that aspirin can effectively inhibit the maturation 
      of DCs. However, the protective effect of aspirin on acute cardiac allograft 
      rejection has not been studied. The aim of this study was to elucidate the effect 
      of aspirin exert on allograft rejection. Methods: The model of MHC-mismatched 
      (BALB/c to B6 mice) heterotopic heart transplantation was established and 
      administered intraperitoneal injection with aspirin. The severity of allograft 
      rejection, transcriptional levels of cytokines, and characteristics of immune 
      cells were assessed. Bone marrow-derived dendritic cells (BMDCs) were generated 
      with or without aspirin. The function of DCs was determined via mixed lymphocyte 
      reaction (MLR). The signaling pathway of DCs was detected by Western blotting. 
      Results: Aspirin significantly prolonged the survival of cardiac allograft in 
      mouse, inhibited the production of pro-inflammatory cytokines and the 
      differentiation of effector T cells (Th1 and Th17), as well as promoted the 
      regulatory T cells (Treg). The maturation of DCs in the spleen was obviously 
      suppressed with aspirin treatment. In vitro, aspirin decreased the activation of 
      NF-kappaB signaling of DCs, as well as impeded MHCII and co-stimulatory molecules 
      (CD80, CD86, and CD40) expression on DCs. Moreover, both the pro-inflammatory 
      cytokines and function of DCs were suppressed by aspirin. Conclusion: Aspirin 
      inhibits the maturation of DCs through the NF-kappaB signaling pathway and attenuates 
      acute cardiac allograft rejection.
CI  - Copyright (c) 2021 Zhang, Chang, Zou, Xu, Cui, Chen, Li, Du, Wu, Yu and Du.
FAU - Zhang, Xi
AU  - Zhang X
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Chang, Aie
AU  - Chang A
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Zou, Yanqiang
AU  - Zou Y
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Xu, Heng
AU  - Xu H
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Cui, Jikai
AU  - Cui J
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Chen, Zhang
AU  - Chen Z
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Du, Yifan
AU  - Du Y
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Wu, Jie
AU  - Wu J
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Yu, Jizhang
AU  - Yu J
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Du, Xinling
AU  - Du X
AD  - Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20210816
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC8415307
OTO - NOTNLM
OT  - NF-kappaB
OT  - allograft rejection
OT  - aspirin
OT  - dendritic cells
OT  - heart transplantation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/09/07 06:00
MHDA- 2021/09/07 06:01
PMCR- 2021/08/16
CRDT- 2021/09/06 05:54
PHST- 2021/05/08 00:00 [received]
PHST- 2021/07/05 00:00 [accepted]
PHST- 2021/09/06 05:54 [entrez]
PHST- 2021/09/07 06:00 [pubmed]
PHST- 2021/09/07 06:01 [medline]
PHST- 2021/08/16 00:00 [pmc-release]
AID - 706748 [pii]
AID - 10.3389/fphar.2021.706748 [doi]
PST - epublish
SO  - Front Pharmacol. 2021 Aug 16;12:706748. doi: 10.3389/fphar.2021.706748. 
      eCollection 2021.