PMID- 34484230 OWN - NLM STAT- MEDLINE DCOM- 20211220 LR - 20211220 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 12 DP - 2021 TI - Gut Bacterial Characteristics of Patients With Type 2 Diabetes Mellitus and the Application Potential. PG - 722206 LID - 10.3389/fimmu.2021.722206 [doi] LID - 722206 AB - Type 2 diabetes mellitus (T2DM) is a complex disorder comprehensively influenced by genetic and environmental risk, and research increasingly has indicated the role of microbial dysbiosis in T2DM pathogenesis. However, studies comparing the microbiome characteristics between T2DM and healthy controls have reported inconsistent results. To further identify and describe the characteristics of the intestinal flora of T2DM patients, we performed a systematic review and meta-analysis of stool microbial profiles to discern and describe microbial dysbiosis in T2DM and to explore heterogeneity among 7 studies (600 T2DM cases, 543 controls, 1143 samples in total). Using a random effects model and a fixed effects model, we observed significant differences in beta diversity, but not alpha diversity, between individuals with T2DM and controls. We identified various operational taxonomic unit (OTUs) and bacterial genera with significant odds ratios for T2DM. The T2DM signatures derived from a single study by stepwise feature selection could be applied in other studies. By training on multiple studies, we improved the detection accuracy and disease specificity for T2DM. We also discuss the relationship between T2DM-enriched or T2DM-depleted genera and probiotics and provide new ideas for diabetes prevention and improvement. CI - Copyright (c) 2021 Que, Cao, He, Zhang, Chen, Yan, Lin, Yang, Wu, Zhu, Chen, Chen, Xiao, Hou and Zhang. FAU - Que, Yanyan AU - Que Y AD - Department of Endocrinology, Zhongshan Hospital Xiamen University, Xiamen, China. FAU - Cao, Man AU - Cao M AD - Department of Mathematics and Numerical Simulation and High-Performance Computing Laboratory, School of Sciences, Nanchang University, Nanchang, China. FAU - He, Jianquan AU - He J AD - Department of Rehabilitation, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China. FAU - Zhang, Qiang AU - Zhang Q AD - Department of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Chen, Qiongyun AU - Chen Q AD - Department of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Yan, Changsheng AU - Yan C AD - Department of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Lin, Aiqiang AU - Lin A AD - Department of Research and Development, Xiamen Treatgut Biotechnology Co., Ltd., Xiamen, China. FAU - Yang, Luxi AU - Yang L AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Wu, Zezhen AU - Wu Z AD - Department of Endocrine and Metabolic Diseases, Longhu Hospital, The First Affiliated Hospital of Medical College of Shantou University, Shantou, China. AD - Graduate School, Medical College of Shantou University, Shantou, China. FAU - Zhu, Dan AU - Zhu D AD - Department of Endocrine and Metabolic Diseases, Longhu Hospital, The First Affiliated Hospital of Medical College of Shantou University, Shantou, China. FAU - Chen, Fengwu AU - Chen F AD - Department of Endocrine and Metabolic Diseases, Longhu Hospital, The First Affiliated Hospital of Medical College of Shantou University, Shantou, China. FAU - Chen, Zhangran AU - Chen Z AD - Department of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Xiao, Chuanxing AU - Xiao C AD - School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China. AD - School of Medicine, Xiamen University, Xiamen, China. FAU - Hou, Kaijian AU - Hou K AD - Department of Endocrine and Metabolic Diseases, Longhu Hospital, The First Affiliated Hospital of Medical College of Shantou University, Shantou, China. FAU - Zhang, Bangzhou AU - Zhang B AD - Department of Gastroenterology, Zhongshan Hospital Xiamen University, Xiamen, China. AD - School of Medicine, Xiamen University, Xiamen, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210812 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Case-Control Studies MH - Diabetes Mellitus, Type 2/*complications/metabolism MH - Dysbiosis/*etiology MH - Feces/microbiology MH - *Gastrointestinal Microbiome MH - Humans MH - Metagenome MH - Metagenomics/methods MH - Probiotics MH - RNA, Ribosomal, 16S MH - ROC Curve PMC - PMC8415158 OTO - NOTNLM OT - 16S rRNA sequencing OT - T2DM OT - meta-analysis OT - microbiota OT - probiotics COIS- AL was employed by company Xiamen Treatgut Biotechnology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/09/07 06:00 MHDA- 2021/12/21 06:00 PMCR- 2021/01/01 CRDT- 2021/09/06 05:55 PHST- 2021/06/08 00:00 [received] PHST- 2021/07/26 00:00 [accepted] PHST- 2021/09/06 05:55 [entrez] PHST- 2021/09/07 06:00 [pubmed] PHST- 2021/12/21 06:00 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2021.722206 [doi] PST - epublish SO - Front Immunol. 2021 Aug 12;12:722206. doi: 10.3389/fimmu.2021.722206. eCollection 2021.