PMID- 34486151
OWN - NLM
STAT- MEDLINE
DCOM- 20211228
LR  - 20211228
IS  - 1521-4028 (Electronic)
IS  - 0233-111X (Linking)
VI  - 61
IP  - 10
DP  - 2021 Oct
TI  - Investigation into the prevalent CRISPR-Cas systems among the Aeromonas genus.
PG  - 874-882
LID - 10.1002/jobm.202100234 [doi]
AB  - Clustered regularly interspaced short palindromic repeats (CRISPR) is a 
      prokaryotic adaptive immune system that checks invasion by mobile genetic 
      elements through nuclease targeting. In this study, we investigated the 
      occurrence, diversity, and features of the CRISPR system in the genus Aeromonas 
      using bioinformatics tools. Only 13 out of 122 complete genomes (10.66%) of the 
      genus Aeromonas from the NCBI GenBank database harbored the CRISPR system. The 
      Type I-F system was the most prevalent CRISPR system among the Aeromonads, 
      followed by the Type I-E system. Only one strain harbored a Type I-C CRISPR 
      system. Among the Aeromonads, Aeromonas caviae (22.7%) and Aeromonas veronii 
      (20%) had a higher prevalence rate of the complete CRISPR system. The analysis of 
      direct repeat (DR) sequences showed that all could form stable RNA secondary 
      structures. A phylogenetic tree generated for the Cas1 protein classified CRISPR 
      subtypes into three distinct clusters. Among the 748 spacers investigated, 41.98% 
      and 17.25% showed perfect homology to phage and plasmid sequences, respectively. 
      Some arrays had duplicated spacers. The CRISPR loci are closely linked to 
      antibiotic resistance genes in most strains. Collectively, our results would 
      contribute to research on antibiotic resistance in the Aeromonas group, and 
      provide new insights into the diversity and evolution of the CRISPR-Cas system.
CI  - (c) 2021 Wiley-VCH GmbH.
FAU - Baliga, Pallavi
AU  - Baliga P
AD  - Department of Aquatic Animal Health Management, College of Fisheries, Karnataka 
      Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, 
      India.
FAU - Shekar, Malathi
AU  - Shekar M
AUID- ORCID: 0000-0001-9193-9416
AD  - Department of Aquatic Animal Health Management, College of Fisheries, Karnataka 
      Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, 
      India.
FAU - Tg, Puneeth
AU  - Tg P
AD  - Department of Aquatic Animal Health Management, College of Fisheries, Karnataka 
      Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, 
      India.
FAU - Sk, Girisha
AU  - Sk G
AD  - Department of Aquatic Animal Health Management, College of Fisheries, Karnataka 
      Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, 
      India.
LA  - eng
GR  - PDF/2018/ 000811/Science and Engineering Research Board, India/
PT  - Journal Article
DEP - 20210906
PL  - Germany
TA  - J Basic Microbiol
JT  - Journal of basic microbiology
JID - 8503885
SB  - IM
MH  - Aeromonas/*genetics
MH  - Bacteriophages/genetics
MH  - *CRISPR-Cas Systems
MH  - Computational Biology/methods
MH  - Genome, Bacterial
MH  - Phylogeny
MH  - Plasmids
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Aeromonas
OT  - CRISPR
OT  - Cas proteins
OT  - direct repeat
OT  - spacer
EDAT- 2021/09/07 06:00
MHDA- 2021/12/29 06:00
CRDT- 2021/09/06 06:28
PHST- 2021/08/12 00:00 [revised]
PHST- 2021/05/11 00:00 [received]
PHST- 2021/08/21 00:00 [accepted]
PHST- 2021/09/07 06:00 [pubmed]
PHST- 2021/12/29 06:00 [medline]
PHST- 2021/09/06 06:28 [entrez]
AID - 10.1002/jobm.202100234 [doi]
PST - ppublish
SO  - J Basic Microbiol. 2021 Oct;61(10):874-882. doi: 10.1002/jobm.202100234. Epub 
      2021 Sep 6.