PMID- 34486259
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210915
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 13
IP  - 5
DP  - 2021 Sep
TI  - Presence of IgE Autoantibodies Against Eosinophil Peroxidase and Eosinophil 
      Cationic Protein in Severe Chronic Spontaneous Urticaria and Atopic Dermatitis.
PG  - 746-761
LID - 10.4168/aair.2021.13.5.746 [doi]
AB  - PURPOSE: Eosinophils are frequently found in atopic dermatitis (AD) and chronic 
      spontaneous urticaria (CSU) that release eosinophil peroxidase (EPX) and 
      eosinophil cationic protein (ECP). Continuous exposure to these proteins could 
      trigger an autoimmune response which may contribute to the pathogenesis and 
      severity of skin inflammation. In this study, we investigate the immunoglobulin E 
      (IgE) response against eosinophil proteins in CSU and AD. METHODS: We recruited 
      patients with severe AD, severe CSU and healthy subjects to explore the presence 
      of IgE autoantibodies and cross-reactivity against EPX, ECP and thyroid 
      peroxidase (TPO). The potential cross-reactive epitopes among the peroxidase 
      family were determined using in silico tools. RESULTS: The frequencies of 
      anti-EPX IgE (28.8%) and anti-ECP IgE (26.6%) were higher in the AD group, and 
      anti-TPO IgE was higher in the CSU group (27.2%). In the CSU group, there was a 
      correlation between the anti-EPX IgE and anti-TPO IgE levels (r = 0.542, P < 
      0.001); TPO inhibited 42% of IgE binding to EPX, while EPX inhibited 59% of IgE 
      binding to TPO, suggesting a cross-reactivity with EPX as a primary sensitizer. 
      There was greater inhibition when we used a pool of sera CSU and AD, TPO 
      inhibited 52% of IgE binding to EPX, while EPX inhibited 78% of IgE binding to 
      TPO. In silico analysis showed a possible shared epitope in the peroxidase 
      protein family. CONCLUSIONS: IgE against eosinophil proteins may contribute to 
      chronic inflammation in patients with AD and CSU. Cross-reactivity between EPX 
      and TPO could explain thyroid problems in CSU patients.
CI  - Copyright (c) 2021 The Korean Academy of Asthma, Allergy and Clinical Immunology * 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Sanchez, Jorge
AU  - Sanchez J
AUID- ORCID: 0000-0001-6341-783X
AD  - Group of Clinical and Experimental Allergy, IPS Universitaria Clinic, University 
      of Antioquia, Medellin, Colombia. jorgem.sanchez@udea.edu.co.
FAU - Sanchez, Andres
AU  - Sanchez A
AUID- ORCID: 0000-0001-7460-3427
AD  - Group of Clinical and Experimental Allergy, IPS Universitaria Clinic, University 
      of Antioquia, Medellin, Colombia.
AD  - Medical Research Group (GINUMED), Rafael Nunez University Corporation, Department 
      Immunology, Faculty of Medicine, Cartagena, Colombia.
FAU - Munera, Marlon
AU  - Munera M
AUID- ORCID: 0000-0003-3428-0541
AD  - Medical Research Group (GINUMED), Rafael Nunez University Corporation, Department 
      Immunology, Faculty of Medicine, Cartagena, Colombia.
FAU - Garcia, Elizabeth
AU  - Garcia E
AUID- ORCID: 0000-0002-7456-4007
AD  - Allergy Department, Faculty of Medicine, Universidad de los Andes, Bogota, 
      Colombia.
AD  - Department Allergology, Fundacion Santa Fe de Bogota, Bogota, Colombia.
FAU - Lopez, Juan-Felipe
AU  - Lopez JF
AUID- ORCID: 0000-0002-9993-9530
AD  - Group of Clinical and Experimental Allergy, IPS Universitaria Clinic, University 
      of Antioquia, Medellin, Colombia.
AD  - Institute for Immunological Research, University of Cartagena, Cartagena, 
      Colombia.
FAU - Velasquez-Lopera, Margarita
AU  - Velasquez-Lopera M
AUID- ORCID: 0000-0001-8604-6488
AD  - Dermatological Research Center, Centro de Investigaciones Dermatologicas 
      (CIDERM), University of Antioquia, Medellin, Colombia.
FAU - Cardona, Ricardo
AU  - Cardona R
AUID- ORCID: 0000-0002-7428-2413
AD  - Group of Clinical and Experimental Allergy, IPS Universitaria Clinic, University 
      of Antioquia, Medellin, Colombia.
LA  - eng
GR  - IPS Universitaria/Colombia
GR  - Unidad Alergologica/Colombia
GR  - San Vicente de Paul/Colombia
GR  - UdeA/Universidad de Antioquia/Colombia
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC8419645
OTO - NOTNLM
OT  - Allergy
OT  - atopic dermatitis
OT  - autoantibodies
OT  - chronic urticaria
OT  - eosinophil cationic protein
OT  - eosinophil peroxidase
OT  - immunoglobulin E
OT  - peroxidase
OT  - thyroperoxidase
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2021/09/07 06:00
MHDA- 2021/09/07 06:01
PMCR- 2021/09/01
CRDT- 2021/09/06 06:38
PHST- 2020/10/04 00:00 [received]
PHST- 2020/12/17 00:00 [revised]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2021/09/06 06:38 [entrez]
PHST- 2021/09/07 06:00 [pubmed]
PHST- 2021/09/07 06:01 [medline]
PHST- 2021/09/01 00:00 [pmc-release]
AID - 13.746 [pii]
AID - 10.4168/aair.2021.13.5.746 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2021 Sep;13(5):746-761. doi: 
      10.4168/aair.2021.13.5.746.