PMID- 34491255 OWN - NLM STAT- MEDLINE DCOM- 20220207 LR - 20220207 IS - 2050-7518 (Electronic) IS - 2050-750X (Linking) VI - 9 IP - 38 DP - 2021 Oct 6 TI - Instant hydrogelation encapsulates drugs onto implants intraoperatively against osteoarticular tuberculosis. PG - 8056-8066 LID - 10.1039/d1tb00997d [doi] AB - Osteoarticular Tuberculosis (TB) is a challenging issue because of its chronicity and recurrence. Many drug delivery systems (DDSs) have been developed for general chemotherapy. Herein, we take advantage of instant hydrogelation to in situ encapsulate drugs onto implants intraoperatively, optimizing the drug release profile against osteoarticular TB. First-line chemodrugs, i.e. rifampicin (RFP) and isoniazid (INH) are firstly loaded on tricalcium phosphate (TCP). Then, the encapsulating hydrogel is fabricated by dipping in chitosan (CS) and beta-glycerophosphate (beta-GP) solution and heating at 80 degrees C for 40 min. The hydrogel encapsulation inhibits explosive drug release initially, but maintains long-term drug release (INH, 158 days; RFP, 53 days) in vitro. Therefore, this technique could inhibit bone destruction and inflammation from TB effectively in vivo, better than our previous ex situ prepared DDSs. The encapsulating technology, i.e. instant hydrogelation of drug-loaded implants, shows potential for regulating the type and ratio of drugs, elastic and viscous modulus of the hydrogel according to the state of illness intraoperatively for optimal drug release. FAU - Li, Yuan AU - Li Y AUID- ORCID: 0000-0002-4588-8977 AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Li, Litao AU - Li L AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Sha, Xiaoling AU - Sha X AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Zhang, Kuo AU - Zhang K AUID- ORCID: 0000-0003-3687-3172 AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. FAU - Li, Guang AU - Li G AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Ma, Yiguang AU - Ma Y AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Zhou, Jin AU - Zhou J AD - CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, P. R. China. FAU - Hao, Yanfei AU - Hao Y AD - The 8th Medical Center of Chinese PLA General Hospital, No. 17 Heishanhu Road, Beijing 100091, China. FAU - Zhang, Zhong AU - Zhang Z AUID- ORCID: 0000-0002-9102-1311 AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. FAU - Cui, Xu AU - Cui X AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Tang, Pei-Fu AU - Tang PF AD - Department of Orthopedic Medicine, The 4th Medical Center of Chinese PLA General Hospital, No. 51 Fucheng Road, Beijing 100000, China. cuixuprossor@163.com. FAU - Wang, Lei AU - Wang L AUID- ORCID: 0000-0003-1405-9815 AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. FAU - Wang, Hao AU - Wang H AUID- ORCID: 0000-0002-1961-0787 AD - CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China. wanglei@nanoctr.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20211006 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 RN - 0 (Antitubercular Agents) RN - 0 (Biocompatible Materials) RN - 0 (Calcium Phosphates) RN - 0 (Drug Carriers) RN - 0 (Glycerophosphates) RN - 0 (Hydrogels) RN - 9012-76-4 (Chitosan) RN - K4C08XP666 (tricalcium phosphate) RN - V83O1VOZ8L (Isoniazid) RN - VJT6J7R4TR (Rifampin) RN - WWH06G87W6 (beta-glycerophosphoric acid) SB - IM MH - Animals MH - Antitubercular Agents/chemistry/metabolism/pharmacology/*therapeutic use MH - Biocompatible Materials/chemistry/pharmacology MH - Calcium Phosphates/chemistry MH - Cell Line MH - Cell Survival/drug effects MH - Chitosan/chemistry MH - Disease Models, Animal MH - Drug Carriers/*chemistry MH - Drug Liberation MH - Femur/pathology MH - Glycerophosphates/chemistry MH - Hydrogels/*chemistry MH - Isoniazid/chemistry/metabolism/pharmacology/therapeutic use MH - Mice MH - Mycobacterium tuberculosis/drug effects/physiology MH - Porosity MH - Prostheses and Implants MH - Rifampin/chemistry/metabolism/pharmacology/therapeutic use MH - Tuberculosis, Osteoarticular/*drug therapy EDAT- 2021/09/08 06:00 MHDA- 2022/02/08 06:00 CRDT- 2021/09/07 12:28 PHST- 2021/09/08 06:00 [pubmed] PHST- 2022/02/08 06:00 [medline] PHST- 2021/09/07 12:28 [entrez] AID - 10.1039/d1tb00997d [doi] PST - epublish SO - J Mater Chem B. 2021 Oct 6;9(38):8056-8066. doi: 10.1039/d1tb00997d.