PMID- 34492318 OWN - NLM STAT- MEDLINE DCOM- 20220128 LR - 20220128 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 282 DP - 2022 Jan 10 TI - Antitumor and hepatoprotective effect of Cuscuta reflexa Roxb. in a murine model of colon cancer. PG - 114597 LID - S0378-8741(21)00826-6 [pii] LID - 10.1016/j.jep.2021.114597 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Cuscuta reflexa Roxb. (C. reflexa) is a well-known traditional herbal plant, with numerous inherent therapeutic potentials including anticancer, antitumor, antibacterial, analgesic, anthelmintic, laxative and others. Moreover, the anticancer and antitumor potentials of this herb are ongoing with several trails, thus an attempt was made to assess the anticancer and hepatoprotective potentials of traditional C. reflexa herbs. METHOD: The dried ethanolic extract of C. reflexa was tested for acute oral toxicity in the treated animals subsequently their behavioral, neurological, and autonomic profiles changes were observed. The preliminary anti-cancer effects of extracts against 1, 2- Dimethyl hydrazine (DMH) induced animals were assessed through barium enema X-ray, colonoscopy, and Aberrant crypt foci (ACF) studies. The blood samples of the animals (treated and untreated) were collected and their in-vitro histological parameters were evaluated by the experienced technician. RESULTS: It was observed that C. reflexa significantly reduced Disease activity indexing (DAI) level and ACF counting, as well as demonstrated similar activity as of the standard drug 5-Fluorouracil (5-FU). Histopathological results revealed that the apoptotic bodies decreased in the DMH-induced group (group II) during cancer progression while in 5-FU treated (group III) and C. reflexa treated (group IV and V) animals the apoptotic bodies were increased. Inversely, the mitotic bodies increased in group II animals and reduced in group III, IV, and V animals. In the colonic section, DMH-induced cancer assay exhibited significant effects on the levels of hemoglobin, Packed cell volume (PCV), Red blood cell (RBC) counts, Mean corpuscular hemoglobin concentration (MCHC), Mean corpuscular volume (MCV), and Mean cell hemoglobin (MCH), and was found to be less in group II animals whereas administration of C. reflexa efficiently recovered back the loss probably by healing the colon damage/depletion of cancer progression. Moreover, compared to the group II animals, the neutrophil count was within the normal range in C. reflexa administered group. CONCLUSIONS: In the present study, the major hematological parameters significantly increased within DMH treated animals and exhibited extensive damage in the hepatic regions. Moreover, the histopathological findings demonstrated that the C. reflexa extracts potentially reduced the cell proliferation, with no toxicity. The C. reflexa extracts exhibited impending anti-cancer activity as well as protected the hepatic cells and thus could be potentially used in the management of colon or colorectal cancer and hepatic impairments. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Mishra, Shobhit AU - Mishra S AD - Amity Institute of Pharmacy, Amity University, Noida, U.P., India. Electronic address: shobhitpharmacist@gmail.com. FAU - Alhodieb, Fahad Saad AU - Alhodieb FS AD - Department of Clinical Nutrition, College of Applied Health Sciences in Arrass, Qassim University, P.O. BOX:6666, Buraidah, 51452, Saudi Arabia. Electronic address: f.alhodieb@qu.edu.sa. FAU - Barkat, Md Abul AU - Barkat MA AD - Department of Pharmaceutics, College of Pharmacy, University of Hafr Al-Batin, Al Jamiah, Hafr Al Batin, 39524, Saudi Arabia. Electronic address: abulbarkat05@gmail.com. FAU - Hassan, Mohd Zaheen AU - Hassan MZ AD - Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha, Saudi Arabia. Electronic address: mzhapharma@gmail.com. FAU - Barkat, Harshita Abul AU - Barkat HA AD - Department of Pharmaceutics, College of Pharmacy, University of Hafr Al-Batin, Al Jamiah, Hafr Al Batin, 39524, Saudi Arabia. Electronic address: harshiab001@gmail.com. FAU - Ali, Raisuddin AU - Ali R AD - Department of Pharmaceutics & Research Center, College of Pharmacy, King Saud University, Saudi Arabia. Electronic address: aliraisuddin786@gmail.com. FAU - Alam, Perwaiz AU - Alam P AD - Department of Pharmaceutical Chemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India. Electronic address: perwaizalam786@gmail.com. FAU - Alam, Ozair AU - Alam O AD - Department of Pharmaceutical Chemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India. Electronic address: dr.ozairalam@gmail.com. LA - eng PT - Journal Article DEP - 20210904 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Antineoplastic Agents, Phytogenic) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Plant Extracts) SB - IM MH - *Aberrant Crypt Foci/drug therapy/pathology MH - Animals MH - Antineoplastic Agents, Phytogenic/pharmacology MH - Behavior, Animal/drug effects MH - Cell Proliferation/drug effects MH - Colon/diagnostic imaging/drug effects/pathology MH - *Colonic Neoplasms/drug therapy/pathology MH - *Cuscuta MH - Drug Monitoring/methods MH - Drugs, Chinese Herbal/pharmacology MH - Hepatocytes/drug effects/pathology MH - Mice MH - Plant Extracts/pharmacology MH - Toxicity Tests/*methods OTO - NOTNLM OT - 1, 2-dimethyl hydrazine OT - Anti cancer OT - Colorectal cancer OT - Cuscuta reflexa Roxb OT - Hepatoprotective OT - Histopathological assay EDAT- 2021/09/08 06:00 MHDA- 2022/01/29 06:00 CRDT- 2021/09/07 20:13 PHST- 2021/05/22 00:00 [received] PHST- 2021/08/16 00:00 [revised] PHST- 2021/09/01 00:00 [accepted] PHST- 2021/09/08 06:00 [pubmed] PHST- 2022/01/29 06:00 [medline] PHST- 2021/09/07 20:13 [entrez] AID - S0378-8741(21)00826-6 [pii] AID - 10.1016/j.jep.2021.114597 [doi] PST - ppublish SO - J Ethnopharmacol. 2022 Jan 10;282:114597. doi: 10.1016/j.jep.2021.114597. Epub 2021 Sep 4.