PMID- 34493934
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220630
IS  - 1573-3149 (Print)
IS  - 1573-3904 (Electronic)
IS  - 1573-3149 (Linking)
VI  - 27
IP  - 4
DP  - 2021
TI  - Computational Design of a Multi-epitope Vaccine Against Clostridium chauvoei: An 
      Immunoinformatics Approach.
PG  - 2639-2649
LID - 10.1007/s10989-021-10279-9 [doi]
AB  - Blackleg is an infectious disease of animals that is commonly caused by 
      Clostridium chauvoei and characterized by localized muscle necrosis. In this 
      study, proteome-mining and immunoinformatics approaches were applied to identify 
      novel antigenic proteins and to construct a multi-epitope vaccine against C. 
      chauvoei. All proteins of C. chauvoei strains were retrieved from the NCBI 
      Microbial Genome Database containing both genomic and proteomic data of 
      prokaryotes. The proteins were analyzed to exclude non-redundant sequences and to 
      determine antigenic, virulent, and non-allergenic vaccine candidates through 
      several online tools, resulting in seven protein candidates. Cytotoxic T and B 
      cell epitopes of these proteins were evaluated through the tools present in the 
      immune epitope database and the prioritized antigenic epitopes were then 
      conjugated via appropriate linkers to construct the vaccine candidate. After the 
      evaluation of physicochemical properties of the construct, the tertiary structure 
      was modeled and refined through trRosetta and GalaxyRefine, respectively. The 
      quality of the 3D structure was validated by ERRAT score, z-score, and 
      Ramachandran plot and the construct was then docked with bovine Toll-like 
      receptor 4 (TLR 4) using ClusPro. The docked complex was subjected to Molecular 
      Mechanics/Generalized Born Surface Area in the HawkDock server and normal mode 
      analysis in the iMODS simulation suite to assess the binding energy and stability 
      of the complex, respectively. Overall, the vaccine construct was found stable and 
      energetically feasible for bovine TLR 4 binding. Therefore, it can be used as a 
      multi-epitope vaccine construct in clostridial vaccines to control the blackleg 
      disease. SUPPLEMENTARY INFORMATION: The online version contains supplementary 
      material available at 10.1007/s10989-021-10279-9.
CI  - (c) The Author(s), under exclusive licence to Springer Nature B.V. 2021.
FAU - Yilmaz Colak, Cigdem
AU  - Yilmaz Colak C
AUID- ORCID: 0000-0002-2107-0855
AD  - Genetic Engineering and Biotechnology Institute, TUBITAK, 41470 Gebze, Kocaeli 
      Turkey. GRID: grid.426409.d. ISNI: 0000 0001 0685 2712
LA  - eng
PT  - Journal Article
DEP - 20210903
PL  - United States
TA  - Int J Pept Res Ther
JT  - International journal of peptide research and therapeutics
JID - 101252761
PMC - PMC8414032
OTO - NOTNLM
OT  - Clostridium chauvoei
OT  - Immunoinformatics
OT  - Multi-epitope vaccine
OT  - Reverse vaccinology
COIS- Conflict of interestThe author declares that there is no conflict of interest.
EDAT- 2021/09/09 06:00
MHDA- 2021/09/09 06:01
PMCR- 2021/09/03
CRDT- 2021/09/08 06:51
PHST- 2021/08/27 00:00 [accepted]
PHST- 2021/09/09 06:00 [pubmed]
PHST- 2021/09/09 06:01 [medline]
PHST- 2021/09/08 06:51 [entrez]
PHST- 2021/09/03 00:00 [pmc-release]
AID - 10279 [pii]
AID - 10.1007/s10989-021-10279-9 [doi]
PST - ppublish
SO  - Int J Pept Res Ther. 2021;27(4):2639-2649. doi: 10.1007/s10989-021-10279-9. Epub 
      2021 Sep 3.