PMID- 34494080
OWN - NLM
STAT- MEDLINE
DCOM- 20220128
LR  - 20220128
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 85
IP  - 11
DP  - 2021 Oct 21
TI  - Overexpression of AGR2vH, an oncogenic AGR2 spliced transcript, potentiates 
      tumorigenicity and proteomic alterations in cholangiocarcinoma cell.
PG  - 2263-2273
LID - 10.1093/bbb/zbab156 [doi]
AB  - The upregulation of anterior gradient 2 (AGR2) has been observed in 
      cholangiocarcinoma (CCA) cells, nras-mutant zebrafish, and specimens derived from 
      CCA patients. Our previous study reported AGR2 splicing into AGR2vH to facilitate 
      CCA cell aggressiveness, while this work aims to investigate the molecular 
      mechanisms underlying AGR2vH. First, AGR2vH upregulation was demonstrated in CCA 
      tissues derived from patients. For in vitro studies, established 
      AGR2vH-overexpressing KKU-213A cells were found to exhibit increased 
      proliferation and clonogenicity. In vivo tumorigenicity assessed in a mouse model 
      represented higher tumorigenic potential in AGR2vH-overexpressing cell xenograft 
      mice. Next, LC-MS/MS was analyzed, indicating that AGR2vH may be associated with 
      CCA cell proliferation via Wnt/beta-catenin signaling pathway activation, which was 
      verified by beta-catenin expression and nuclear translocation. The current results 
      provide evidence that AGR2vH upregulation promotes tumorigenicity in CCA cells 
      linked with an alteration of CCA cell proteome.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of Japan 
      Society for Bioscience, Biotechnology, and Agrochemistry.
FAU - Yosudjai, Juthamas
AU  - Yosudjai J
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
FAU - Inpad, Chaturong
AU  - Inpad C
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
FAU - Pothipan, Phattarin
AU  - Pothipan P
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
FAU - Saisomboon, Saowaluk
AU  - Saisomboon S
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Surangkul, Damrasamon
AU  - Surangkul D
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
FAU - Phimsen, Suchada
AU  - Phimsen S
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
FAU - Hongsrichan, Nuttanan
AU  - Hongsrichan N
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
AD  - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Wongkham, Sopit
AU  - Wongkham S
AUID- ORCID: 0000-0001-9578-3041
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Jirawatnotai, Siwanon
AU  - Jirawatnotai S
AD  - Siriraj Center of Research for Excellence for Systems Pharmacology, Department of 
      Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 
      Thailand.
FAU - Roytrakul, Sittiruk
AU  - Roytrakul S
AD  - National Center for Genetic Engineering and Biotechnology, National Science and 
      Technology Development Agency, Pathumthani, Thailand.
FAU - Kaewkong, Worasak
AU  - Kaewkong W
AUID- ORCID: 0000-0002-7130-5827
AD  - Department of Biochemistry, Faculty of Medical Science, Naresuan University, 
      Phitsanulok, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
LA  - eng
GR  - Naresuan University/
GR  - Thailand Research Fund/
GR  - TRF-MRG6080014/Office of the Higher Education Commission/
GR  - National Research Council of Thailand/
GR  - NRCT5-RSA63011-04/NRCT-Research Career Development/
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
SB  - IM
MH  - *Proteomics
OTO - NOTNLM
OT  - AGR2
OT  - alternative splicing
OT  - cholangiocarcinoma
OT  - proteomics
OT  - tumorigenesis
EDAT- 2021/09/09 06:00
MHDA- 2022/01/29 06:00
CRDT- 2021/09/08 06:55
PHST- 2021/07/08 00:00 [received]
PHST- 2021/09/02 00:00 [accepted]
PHST- 2021/09/09 06:00 [pubmed]
PHST- 2022/01/29 06:00 [medline]
PHST- 2021/09/08 06:55 [entrez]
AID - 6366227 [pii]
AID - 10.1093/bbb/zbab156 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2021 Oct 21;85(11):2263-2273. doi: 
      10.1093/bbb/zbab156.