PMID- 34494438
OWN - NLM
STAT- MEDLINE
DCOM- 20210923
LR  - 20210923
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 37
DP  - 2021 Sep 22
TI  - Enhanced Bio-Barcode Immunoassay Using Droplet Digital PCR for Multiplex 
      Detection of Organophosphate Pesticides.
PG  - 11131-11141
LID - 10.1021/acs.jafc.1c03216 [doi]
AB  - A bio-barcode immunoassay based on droplet digital polymerase chain reaction 
      (ddPCR) was developed to simultaneously quantify triazophos, parathion, and 
      chlorpyrifos in apple, cucumber, cabbage, and pear. Three gold nanoparticle 
      (AuNP) probes and magnetic nanoparticle (MNP) probes were prepared, binding 
      through their antibodies with the three pesticides in the same tube. Three groups 
      of primers, probes, templates, and three antibodies were designed to ensure the 
      specificity of the method. Under the optimal conditions, the detection limits 
      (expressed as IC(10)) of triazophos, parathion, and chlorpyrifos were 0.22, 0.45, 
      and 4.49 ng mL(-1), respectively. The linear ranges were 0.01-20, 0.1-100, and 
      0.1-500 ng mL(-1), and the correlation coefficients (R(2)) were 0.9661, 0.9834, 
      and 0.9612, respectively. The recoveries and relative standard deviations (RSDs) 
      were in the ranges of 75.5-98.9 and 8.3-16.7%. This study provides the first 
      insights into the ddPCR for the determination of organophosphate pesticides. It 
      also laid the foundation for high-throughput detection of other small molecules.
FAU - Cui, Xueyan
AU  - Cui X
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
AD  - State Key Laboratory of Biobased Material and Green Papermaking, College of Food 
      Science and Engineering, Qilu University of Technology, Shandong Academy of 
      Science, Jinan 250353, P. R. China.
AD  - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 
      12211 Giza, Egypt.
AD  - Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum 
      25240, Turkey.
FAU - Zhang, Chan
AU  - Zhang C
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Xu, Lingyuan
AU  - Xu L
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Liu, Haijin
AU  - Liu H
AD  - Inspection and Testing Center of Agricultural and Livestock Products of Tibet, 
      Lhasa 850000, P. R. China.
FAU - Jia, Huiyan
AU  - Jia H
AD  - Ningbo Academy of Agricultural Sciences, Ningbo 315040, Zhengjiang, P. R. China.
FAU - Wang, Yuanshang
AU  - Wang Y
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Cao, Zhen
AU  - Cao Z
AUID- ORCID: 0000-0003-1259-6107
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Salvador, J-Pablo
AU  - Salvador JP
AUID- ORCID: 0000-0002-3608-0634
AD  - Nanobiotechnology for Diagnostics Group, Instituto de Quimica Avanzada de 
      Cataluna, IQAC-CSIC, C/ Jordi Girona, 18-26, 08034 Barcelona, Spain.
AD  - CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Avenida 
      Monforte de Lemos, 3-5, Pavillion 11, Floor 0, 28029 Madrid, Spain.
FAU - She, Yongxin
AU  - She Y
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Jin, Fen
AU  - Jin F
AUID- ORCID: 0000-0002-8686-7582
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Jin, Maojun
AU  - Jin M
AUID- ORCID: 0000-0002-9102-8572
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Science, Beijing 100081, P. R. China.
FAU - Hammock, Bruce D
AU  - Hammock BD
AUID- ORCID: 0000-0003-1408-8317
AD  - Department of Entomology & Nematology and the UC Davis Comprehensive Cancer 
      Center, University of California, Davis, Davis, California 95616, United States.
LA  - eng
PT  - Journal Article
DEP - 20210908
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Pesticides)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Gold
MH  - Immunoassay
MH  - Limit of Detection
MH  - *Metal Nanoparticles
MH  - *Pesticides/analysis
MH  - Polymerase Chain Reaction
OTO - NOTNLM
OT  - ddPCR
OT  - gold nanoparticles
OT  - magnetic nanoparticles
OT  - oligonucleotides
OT  - organophosphate pesticides
EDAT- 2021/09/09 06:00
MHDA- 2021/09/24 06:00
CRDT- 2021/09/08 08:38
PHST- 2021/09/09 06:00 [pubmed]
PHST- 2021/09/24 06:00 [medline]
PHST- 2021/09/08 08:38 [entrez]
AID - 10.1021/acs.jafc.1c03216 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Sep 22;69(37):11131-11141. doi: 10.1021/acs.jafc.1c03216. 
      Epub 2021 Sep 8.