PMID- 34499009
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20231213
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Dec
TI  - The circular RNA circ0005654 interacts with specificity protein 1 via 
      microRNA-363 sequestration to promote gastric cancer progression.
PG  - 6305-6317
LID - 10.1080/21655979.2021.1971031 [doi]
AB  - Circular RNAs (circRNAs), a group of unique long noncoding RNAs, are involved in 
      gastric carcinogenesis through multiple mechanisms, including interacting with 
      microRNAs (miRNAs). Here, circ0005654, significantly upregulated in gastric 
      cancer (GC), was chosen for further examination. circ0005654 was analyzed by 
      RT-qPCR. The function of circ0005654 in GC cells was substantiated by 
      loss-of-function assays. The mechanism of circ0005654 on miR-363/specificity 
      protein 1 (sp1) axis was evaluated in GC cells by bioinformatics analysis, 
      luciferase reporter, FISH, and ChIP assays. We observed that circ0005654 was 
      enhanced in GC tissues and cells. Overexpression of circ0005654 was correlated 
      with a poor long-term prognosis in patients with GC. Functionally, silencing of 
      circ0005654 remarkably suppressed GC cell proliferation, migration and 
      invasiveness in vitro and tumorigenesis and metastases in vivo. It was also 
      established that circ0005654 served as a miR-363 sponge and enhanced sp1 
      expression. Furthermore, sp1 promoted GC carcinogenesis by regulating myc 
      transcription to potentiate the Wnt/beta-catenin pathway. In conclusion, circ0005654 
      expedites the GC development via miR-363/sp1/myc/Wnt/beta-catenin axis and is a new 
      biomarker for GC treatment regimen.
FAU - Yang, Cui
AU  - Yang C
AD  - Department of Clinical Medicine, Wanxi Health Vocational College, Lu'an, Anhui, 
      P.R. China.
FAU - Han, Shengjin
AU  - Han S
AD  - Department of Emergency Surgery, Lu'an People's Hospital, Lu'an, Anhui, P.R. 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MIRN363 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (SP1 protein, human)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - MicroRNAs/*genetics/metabolism
MH  - RNA, Circular/*genetics/metabolism
MH  - Sp1 Transcription Factor/*genetics/metabolism
MH  - Stomach/pathology
MH  - *Stomach Neoplasms/genetics/metabolism/mortality/pathology
MH  - Wnt Signaling Pathway/genetics
PMC - PMC8806801
OTO - NOTNLM
OT  - /beta-catenin pathway
OT  - Circ0005654
OT  - gastric cancer
OT  - microRNA-363
OT  - myc
OT  - sp1
COIS- The authors declare that there are no competing interests in this study.
EDAT- 2021/09/10 06:00
MHDA- 2022/01/14 06:00
PMCR- 2021/09/09
CRDT- 2021/09/09 12:17
PHST- 2021/09/09 12:17 [entrez]
PHST- 2021/09/10 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2021/09/09 00:00 [pmc-release]
AID - 1971031 [pii]
AID - 10.1080/21655979.2021.1971031 [doi]
PST - ppublish
SO  - Bioengineered. 2021 Dec;12(1):6305-6317. doi: 10.1080/21655979.2021.1971031.