PMID- 34502309
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20211018
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 17
DP  - 2021 Aug 30
TI  - Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine 
      Pericardium.
LID - 10.3390/ijms22179406 [doi]
LID - 9406
AB  - Skeletal muscles represent 40% of body mass and its native regenerative capacity 
      can be permanently lost after a traumatic injury, congenital diseases, or tumor 
      ablation. The absence of physiological regeneration can hinder muscle repair 
      preventing normal muscle tissue functions. To date, tissue engineering (TE) 
      represents one promising option for treating muscle injuries and wasting. In 
      particular, hydrogels derived from the decellularized extracellular matrix (dECM) 
      are widely investigated in tissue engineering applications thanks to their 
      essential role in guiding muscle regeneration. In this work, the myogenic 
      potential of dECM substrate, obtained from decellularized bovine pericardium 
      (Tissuegraft Srl), was evaluated in vitro using C2C12 murine muscle cells. To 
      assess myotubes formation, the width, length, and fusion indexes were measured 
      during the differentiation time course. Additionally, the ability of dECM to 
      support myogenesis was assessed by measuring the expression of specific myogenic 
      markers: alpha-smooth muscle actin (alpha-sma), myogenin, and myosin heavy chain (MHC). 
      The results obtained suggest that the dECM niche was able to support and enhance 
      the myogenic potential of C2C12 cells in comparison of those grown on a plastic 
      standard surface. Thus, the use of extracellular matrix proteins, as biomaterial 
      supports, could represent a promising therapeutic strategy for skeletal muscle 
      tissue engineering.
FAU - Carton, Flavia
AU  - Carton F
AUID- ORCID: 0000-0001-6310-9079
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Di Francesco, Dalila
AU  - Di Francesco D
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Fusaro, Luca
AU  - Fusaro L
AD  - Tissuegraft Srl, 28100 Novara, Italy.
FAU - Zanella, Emma
AU  - Zanella E
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Apostolo, Claudio
AU  - Apostolo C
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Oltolina, Francesca
AU  - Oltolina F
AUID- ORCID: 0000-0002-0613-6166
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Cotella, Diego
AU  - Cotella D
AUID- ORCID: 0000-0003-2714-4744
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Prat, Maria
AU  - Prat M
AUID- ORCID: 0000-0003-0262-9729
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
FAU - Boccafoschi, Francesca
AU  - Boccafoschi F
AUID- ORCID: 0000-0002-6734-5447
AD  - Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", 
      28100 Novara, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210830
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Hydrogels)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Cell Differentiation
MH  - Extracellular Matrix/*physiology
MH  - Hydrogels/chemistry
MH  - Mice
MH  - *Muscle Development
MH  - Myoblasts/*cytology
MH  - Pericardium/*cytology
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/chemistry
PMC - PMC8431302
OTO - NOTNLM
OT  - decellularized pericardium
OT  - extracellular matrix
OT  - myogenic differentiation
OT  - skeletal muscle
OT  - tissue engineering
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/11 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/08/30
CRDT- 2021/09/10 01:07
PHST- 2021/07/23 00:00 [received]
PHST- 2021/08/18 00:00 [revised]
PHST- 2021/08/23 00:00 [accepted]
PHST- 2021/09/10 01:07 [entrez]
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/08/30 00:00 [pmc-release]
AID - ijms22179406 [pii]
AID - ijms-22-09406 [pii]
AID - 10.3390/ijms22179406 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Aug 30;22(17):9406. doi: 10.3390/ijms22179406.