PMID- 34503504
OWN - NLM
STAT- MEDLINE
DCOM- 20211105
LR  - 20211105
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 17
IP  - 1
DP  - 2021 Sep 9
TI  - Molecular characterization and immune functional analysis of IRF2 in common carp 
      (Cyprinus carpio L.): different regulatory role in the IFN and NF-kappaB signalling 
      pathway.
PG  - 303
LID - 10.1186/s12917-021-03012-7 [doi]
LID - 303
AB  - BACKGROUND: Interferon regulatory factor 2 (IRF2) is an important transcription 
      factor, which can regulate the IFN response and plays a role in antiviral innate 
      immunity in teleost. RESULTS: In the present study, the full-length cDNA sequence 
      of IRF2 (CcIRF2) was characterized in common carp (Cyprinus carpio L.), which 
      encoded a protein containing a conserved DNA-binding domain (DBD) and an 
      IRF-associated domain (IAD). Phylogenetic analysis showed that CcIRF2 was most 
      closely related with IRF2 of Ctenopharyngodon idella. CcIRF2 transcripts were 
      detectable in all examined tissues, with higher expression in the gills, spleen 
      and brain. CcIRF2 expression was upregulated in immune-related tissues of common 
      carp upon polyinosinic:polycytidylic acid (poly (I:C)) and Aeromonas hydrophila 
      stimulation and induced by poly (I:C), lipopolysaccharide (LPS), peptidoglycan 
      (PGN) and flagellin in the peripheral blood leucocytes (PBLs) and head kidney 
      leukocytes (HKLs). In addition, overexpression of CcIRF2 decreased the expression 
      of IFN and IFN-stimulated genes (ISGs), and a dual-luciferase reporter assay 
      revealed that CcIRF2 could increase the activation of NF-kappaB. CONCLUSIONS: These 
      results indicate that CcIRF2 participates in antiviral and antibacterial immune 
      response and negatively regulates the IFN response, which provide a new insight 
      into the regulation of IFN system in common carp, and are helpful for the 
      prevention and control of infectious diseases in carp farming.
CI  - (c) 2021. The Author(s).
FAU - Li, Hua
AU  - Li H
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China. lihua@sdnu.edu.cn.
FAU - Chen, Xinping
AU  - Chen X
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Zhu, Yaoyao
AU  - Zhu Y
AD  - College of Fisheries and Life Science, Hainan Tropical Ocean University, No. 1 
      Yucai Road, Sanya, 572022, China.
FAU - Liu, Rongrong
AU  - Liu R
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Zheng, Linlin
AU  - Zheng L
AD  - Jinan Eco-environmental Monitoring Center of Shandong Province, No. 17199 Lvyou 
      Road, Jinan, 250101, China.
FAU - Shan, Shijuan
AU  - Shan S
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Zhang, Fumiao
AU  - Zhang F
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - An, Liguo
AU  - An L
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Yang, Guiwen
AU  - Yang G
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China. yanggw@sdnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210909
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Interferon Regulatory Factor-2)
RN  - 0 (NF-kappa B)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Carps/*genetics/*immunology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/immunology
MH  - Interferon Regulatory Factor-2/*genetics/*immunology
MH  - Interferons/*immunology
MH  - NF-kappa B/*immunology
MH  - Signal Transduction/*immunology
PMC - PMC8428054
OTO - NOTNLM
OT  - Aeromonas hydrophila
OT  - Common carp (Cyprinus carpio L.)
OT  - IFN
OT  - Interferon regulatory factor 2 (IRF2)
OT  - NF-kappaB
OT  - Poly (I:C)
COIS- The authors declare that they have no competing interests.
EDAT- 2021/09/11 06:00
MHDA- 2021/11/06 06:00
PMCR- 2021/09/09
CRDT- 2021/09/10 05:34
PHST- 2021/06/08 00:00 [received]
PHST- 2021/09/02 00:00 [accepted]
PHST- 2021/09/10 05:34 [entrez]
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2021/11/06 06:00 [medline]
PHST- 2021/09/09 00:00 [pmc-release]
AID - 10.1186/s12917-021-03012-7 [pii]
AID - 3012 [pii]
AID - 10.1186/s12917-021-03012-7 [doi]
PST - epublish
SO  - BMC Vet Res. 2021 Sep 9;17(1):303. doi: 10.1186/s12917-021-03012-7.