PMID- 34504588
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210911
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 4
DP  - 2021 Oct
TI  - Knockdown of LINC00657 inhibits the viability, migration and invasion of 
      pancreatic cancer cells by regulating the miR-520h/CKS1B axis.
PG  - 1142
LID - 10.3892/etm.2021.10576 [doi]
LID - 1142
AB  - Long non-coding RNA LINC00657 has a critical role in multiple cancers. The aim of 
      the present study was to investigate the regulatory effect of LINC00657 in 
      pancreatic cancer (PC) and reveal its molecular mechanism of function. The 
      expression levels of LINC00657 and microRNA (miR)-520h were detected by reverse 
      transcription-quantitative PCR in PC tissues and cell lines. MTT, wound healing 
      and Transwell assays were used to detect cell viability, migration and invasion, 
      respectively. Dual-luciferase reporter assay was utilized to examine the 
      relationship between LINC00657 and miR-520h and that between miR-520h and 
      cyclin-dependent kinases regulatory subunit 1 (CKS1B). Western blotting was 
      performed to detect CKS1B expression. The expression levels of LINC00657 and 
      CKS1B were enhanced and miR-520h expression level was reduced in PC tissues and 
      cell lines compared with adjacent normal tissues or HPDE6 cells. LINC00657 
      knockdown decreased the viability, migration and invasion of PC cells. 
      Additionally, LINC00657 targeted miR-520h and negatively modulated miR-520h 
      expression. Furthermore, miR-520h overexpression inhibited the viability, 
      migration and invasion of PC cells. In addition, miR-520h targeted CKS1B and 
      reversely regulated CKS1B expression. miR-520h inhibition and CKS1B 
      overexpression alleviated the inhibition effect of LINC00657 knockdown on the 
      viability, migration and invasion of PACA-2 PC cells. In conclusion, the results 
      of the present study demonstrated that LINC00657 knockdown repressed the 
      viability, migration and invasion of PC cells via targeting the miR-520h/CKS1B 
      axis, which may offer a future target for PC therapy.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Li, Peng
AU  - Li P
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
FAU - Wang, Hongsheng
AU  - Wang H
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
FAU - Tang, Ying
AU  - Tang Y
AD  - Department of Nursing, Affiliated Hospital of Beihua University, Jilin, Jilin 
      132011, P.R. China.
FAU - Sun, Shuo
AU  - Sun S
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
FAU - Ma, Yue
AU  - Ma Y
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
FAU - Xu, Yansong
AU  - Xu Y
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
FAU - Chen, Guangyu
AU  - Chen G
AD  - Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Beihua 
      University, Jilin, Jilin 132011, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210809
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8393733
OTO - NOTNLM
OT  - LINC00657
OT  - cyclin-dependent kinases regulatory subunit 1
OT  - microRNA-520h
OT  - pancreatic cancer
COIS- The authors declare that they have no competing interests.
EDAT- 2021/09/11 06:00
MHDA- 2021/09/11 06:01
PMCR- 2021/08/09
CRDT- 2021/09/10 07:00
PHST- 2020/09/18 00:00 [received]
PHST- 2021/05/13 00:00 [accepted]
PHST- 2021/09/10 07:00 [entrez]
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2021/09/11 06:01 [medline]
PHST- 2021/08/09 00:00 [pmc-release]
AID - ETM-0-0-10576 [pii]
AID - 10.3892/etm.2021.10576 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Oct;22(4):1142. doi: 10.3892/etm.2021.10576. Epub 2021 Aug 9.