PMID- 34504909
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220426
IS  - 2352-3204 (Electronic)
IS  - 2352-3204 (Linking)
VI  - 18
DP  - 2021 Dec
TI  - In vitro effects of conditioned medium from bioreactor cultured human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) on skin-derived cell lines.
PG  - 281-291
LID - 10.1016/j.reth.2021.08.003 [doi]
AB  - INTRODUCTION: When stem cells are grafted into tissues, they differentiate and 
      form specialized cells. However, the proficiency of stem cells to endure and 
      assimilate the host cell is dependent on various growth factors and cytokines. 
      According to various studies, these factors are available in the spent media of 
      harvested stem cells, which can be used for treatment in regenerative medicine 
      and cosmetic products. There are differences in cytokine secretion depending on 
      the culture environment, which are clarified in this paper. METHODS: Human 
      umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were cultured either in 
      a bioreactor or in a flask. The conditioned medium from the hUC-MSC cultures in 
      the flask and in the bioreactor was designated as "FM" and "BM", respectively. We 
      assessed the effects of FM and BM on UVB-induced oxidative stress, anti-aging, 
      and melanogenic properties. The amount of growth factors, cell viability, 
      hyaluronic acid (HA), pro-collagen, and pro-melanin were quantitatively evaluated 
      in the FM and BM treated groups. The induction of HA and collagen synthesis was 
      measured in CCD-986SK cells. For melanogenesis, the effects of FM and BM on 
      melanin content and tyrosinase activity were measured in SK-MEL-31 cells. 
      RESULTS: In the present study, the secretion of growth factors, HA, and 
      pro-collagen was significantly higher in the BM treatment, compared to that in 
      the FM treatment. BM protected CCD-986SK cells against death from UVB induced 
      oxidative stress. BM increased the promoter activity of the anti-oxidant genes 
      SOD1, CAT, and GP; and downregulated the accelerating collagen decomposition 
      gene, MMP-1, induced by UVB irradiation. In alpha-melanocyte-stimulating hormone 
      (alpha-MSH) stimulated SK-MEL-31 cells, BM reduced melanin production and decreased 
      the levels of MITF, tyrosinase, TRP-1, and TRP-2. These results suggest that BM 
      could be used as a skin protection agent, because of its anti-apoptotic, 
      anti-aging, and anti-melanogenic properties. This could be attributed to the 
      differences in culturing methods; it is difficult to maintain the temperature and 
      sterility in FM culture, when compared to that in the automated culturing 
      conditions of the BM system. CONCLUSIONS: Collectively, our results indicate that 
      using BM-conditioned hUC-MSC medium is very efficient process for producing raw 
      materials for developing functional cosmetics.
CI  - (c) 2021 The Japanese Society for Regenerative Medicine. Production and hosting by 
      Elsevier B.V.
FAU - Park, Yu Mi
AU  - Park YM
AD  - CHA Advanced Research Institute, 335, Pangyo-ro, Bundang-gu, Seongnam-si, 
      Gyeonggi-do, 13488, Republic of Korea.
AD  - Cell Therapy R&D Center, HansBiomed Corp., 7, Jeongui-ro 8-gil, Songpa-gu, Seoul, 
      05836, Republic of Korea.
FAU - Lee, MinJi
AU  - Lee M
AD  - Cell Therapy R&D Center, HansBiomed Corp., 7, Jeongui-ro 8-gil, Songpa-gu, Seoul, 
      05836, Republic of Korea.
FAU - Jeon, SungHyun
AU  - Jeon S
AD  - R&D Center, HansBiomed Copr., 64, Yuseong-daero 1628, Yuseong-gu, Daejeon, 34054, 
      Republic of Korea.
FAU - Hruzova, Dagmar
AU  - Hruzova D
AD  - Primecell Advanced Therapy, A. S. Jachymova 26/2, 110 00, Prague 1, 60200, Czech 
      Republic.
LA  - eng
PT  - Journal Article
DEP - 20210824
PL  - Netherlands
TA  - Regen Ther
JT  - Regenerative therapy
JID - 101709085
PMC - PMC8390454
OTO - NOTNLM
OT  - Anti-aging
OT  - Anti-apoptosis
OT  - Growth factors
OT  - Human stem cell-conditioned medium
OT  - Hyaluronic acid
OT  - Melanogenesis
COIS- None.
EDAT- 2021/09/11 06:00
MHDA- 2021/09/11 06:01
PMCR- 2021/08/24
CRDT- 2021/09/10 07:04
PHST- 2021/05/21 00:00 [received]
PHST- 2021/07/30 00:00 [revised]
PHST- 2021/08/09 00:00 [accepted]
PHST- 2021/09/10 07:04 [entrez]
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2021/09/11 06:01 [medline]
PHST- 2021/08/24 00:00 [pmc-release]
AID - S2352-3204(21)00062-6 [pii]
AID - 10.1016/j.reth.2021.08.003 [doi]
PST - epublish
SO  - Regen Ther. 2021 Aug 24;18:281-291. doi: 10.1016/j.reth.2021.08.003. eCollection 
      2021 Dec.