PMID- 34505520
OWN - NLM
STAT- MEDLINE
DCOM- 20220309
LR  - 20220309
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Print)
IS  - 0862-8408 (Linking)
VI  - 70
IP  - 5
DP  - 2021 Nov 29
TI  - Genistein does not inhibit TGF-beta1-induced conversion of human dermal 
      fibroblasts to myofibroblasts.
PG  - 815-820
AB  - Transforming growth factor beta 1 (TGF-beta1) is a pro-fibrotic cytokine with a 
      key role in wound repair and regeneration, including induction of 
      fibroblast-to-myofibroblast transition. Genistein is a naturally occurring 
      selective estrogen receptor modulator with promising anti-fibrotic properties. In 
      the present study we aimed to investigate whether genistein modulates TGF-beta1 
      (canonical and non-canonical) signaling in normal dermal fibroblasts at the 
      protein level (Western blot and immunofluorescence). We demonstrated that 
      TGF-beta1 induces the myofibroblast-like phenotype in the studied fibroblast 
      signaling via canonical (SMAD) and non-canonical (AKT, ERK1/2, ROCK) pathways. 
      Genistein induced only ERK1/2 expression, whereas the combination of TGF-beta1 
      and genistein attenuated the ERK1/2 and ROCK signaling. Of note, the other 
      studied pathways remained almost unaffected. From this point of view, genistein 
      does not impair conversion of normal fibroblasts to myofibroblast-like cells.
FAU - Kanuchova, M
AU  - Kanuchova M
AD  - Department of Pharmacology, MediPark and East-Slovak Institute of Cardiovascular 
      Diseases, Kosice, Slovak Republic. galovci@yahoo.com or pgal@vusch.sk.
FAU - Urban, L
AU  - Urban L
FAU - Melegova, N
AU  - Melegova N
FAU - Coma, M
AU  - Coma M
FAU - Dvorankova, B
AU  - Dvorankova B
FAU - Smetana, Karel Jr
AU  - Smetana K Jr
FAU - Gal, P
AU  - Gal P
LA  - eng
PT  - Journal Article
DEP - 20210910
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Phytoestrogens)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - DH2M523P0H (Genistein)
SB  - IM
MH  - Drug Evaluation, Preclinical
MH  - Fibroblasts/*drug effects/metabolism
MH  - Genistein/*pharmacology
MH  - Humans
MH  - Phytoestrogens/*pharmacology
MH  - Primary Cell Culture
MH  - Signal Transduction/drug effects
MH  - Transforming Growth Factor beta1/antagonists & inhibitors/*metabolism
PMC - PMC8820530
COIS- Conflict of Interest There is no conflict of interest.
EDAT- 2021/09/11 06:00
MHDA- 2022/03/11 06:00
PMCR- 2021/09/10
CRDT- 2021/09/10 08:36
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2021/09/10 08:36 [entrez]
PHST- 2021/09/10 00:00 [pmc-release]
AID - 934666 [pii]
AID - pr70_815 [pii]
AID - 10.33549/physiolres.934666 [doi]
PST - ppublish
SO  - Physiol Res. 2021 Nov 29;70(5):815-820. doi: 10.33549/physiolres.934666. Epub 
      2021 Sep 10.