PMID- 34507253
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20220228
IS  - 1532-2238 (Electronic)
IS  - 1096-6374 (Linking)
VI  - 60-61
DP  - 2021 Oct-Dec
TI  - GHRH expression plasmid improves osteoporosis and skin damage in aged mice.
PG  - 101429
LID - S1096-6374(21)00052-6 [pii]
LID - 10.1016/j.ghir.2021.101429 [doi]
AB  - The hormone secretion of GHRH-GH-IGF-1 axis in animals was decreased as aging. 
      These hormones play an important role in maintaining bone mass and bone 
      structure, and also affect the normal structure and function of the skin. We used 
      plasmid-based technology to deliver growth hormone releasing hormone (GHRH) to 
      elderly mice. In the current study, 80 and 120 mug/kg pVAX-GHRH plasmid expression 
      plasmid were injected into old mice, the serum GHRH and insulin-like growth 
      factor-1(IGF-1) content were increased within three weeks (P < 0.05). In the 
      groups of 80 and 120 mug/kg plasmid, the content of procollagen type I N-terminal 
      pro-peptide (PINP) in the serum was increased(P < 0.05), and the content of 
      C-terminal telopeptides of type I collagen (CTX-1) in the serum was reduced 
      significantly (P < 0.05). Furthermore, the expression of osteoprotegerin (OPG) 
      and osteocalcin (OCN) in the femur also was increased(P < 0.05). The bone mineral 
      density(BMD)、trabecular bone volume (BV/TV) and trabecular number(Tb.N) of mouse 
      femur were increased significantly (P < 0.05) and trabecular separation(Tb.Sp) 
      was decreased(P < 0.05). There were more trabecular bones in the bone marrow 
      cavity and the trabecular bones are thicker in the groups of 80 and 120 mug/kg 
      plasmid relative to control. The superoxide dismutase (SOD) content in the skin 
      was increased(P < 0.05), and the malondialdehyde (MDA) content was reduced 
      significantly (P < 0.05). Meanwhile, the skin moisture content also increased 
      significantly(P < 0.05). Moreover, the expression of matrix metalloproteinase 
      3(MMP3) and matrix metalloproteinase 9(MMP9) was decreased in the skin(P < 0.05). 
      The thickness of the dermis and epidermis of the skin had increased 
      significantly(P < 0.05). Skin structure is more dense and complete in the two 
      groups. These results indicate that 80 and 120 mug/kg plasmid-mediated GHRH 
      supplementation can improve osteoporosis and skin aging in aged mice.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Ye, Rui
AU  - Ye R
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Wang, Hai-Long
AU  - Wang HL
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Zeng, De-Wei
AU  - Zeng DW
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Chen, Ting
AU  - Chen T
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Sun, Jia-Jie
AU  - Sun JJ
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Xi, Qian-Yun
AU  - Xi QY
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China.
FAU - Zhang, Yong-Liang
AU  - Zhang YL
AD  - Guangdong Province Key Laboratory of Animal Nutritional Regulation, 483 Wushan 
      Road, Tianhe District, Guangzhou, Guangdong 510642, China; National Engineering 
      Research Center for Breeding Swine Industry, College of Animal Science, South 
      China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 
      Guangdong 510642, China. Electronic address: zhangyl@scau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210904
PL  - Scotland
TA  - Growth Horm IGF Res
JT  - Growth hormone & IGF research : official journal of the Growth Hormone Research 
      Society and the International IGF Research Society
JID - 9814320
RN  - 0 (Hormones)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Bone Density
MH  - Female
MH  - Growth Hormone-Releasing Hormone/*administration & dosage/genetics/metabolism
MH  - Hormones/*administration & dosage/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoporosis/*drug therapy/metabolism/pathology
MH  - Plasmids/*administration & dosage/genetics
MH  - Skin Diseases/*prevention & control
OTO - NOTNLM
OT  - Aged mice
OT  - GHRH
OT  - Osteoporosis
OT  - Plasmid
OT  - Skin aging
EDAT- 2021/09/11 06:00
MHDA- 2022/03/01 06:00
CRDT- 2021/09/10 20:29
PHST- 2021/03/24 00:00 [received]
PHST- 2021/09/01 00:00 [revised]
PHST- 2021/09/03 00:00 [accepted]
PHST- 2021/09/11 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/09/10 20:29 [entrez]
AID - S1096-6374(21)00052-6 [pii]
AID - 10.1016/j.ghir.2021.101429 [doi]
PST - ppublish
SO  - Growth Horm IGF Res. 2021 Oct-Dec;60-61:101429. doi: 10.1016/j.ghir.2021.101429. 
      Epub 2021 Sep 4.