PMID- 34511272
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220301
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 82
IP  - 12
DP  - 2021 Dec
TI  - Pattern recognition receptor expression and maturation profile of dendritic cell 
      subtypes in human tonsils and lymph nodes.
PG  - 976-981
LID - S0198-8859(21)00206-8 [pii]
LID - 10.1016/j.humimm.2021.08.007 [doi]
AB  - Dendritic cells (DCs) with capacity of antigen cross-presentation are of key 
      interest for immunotherapy against cancer as they can induce antigen-specific 
      cytotoxic T lymphocyte (CTL) responses. This study describes frequencies of DC 
      subtypes in human tonsils and lymph nodes, and phenotypic aspects that may be 
      targeted by adjuvant measures. From human tonsils and neck lymph nodes, DCs were 
      identified through flow cytometry, and subsets of plasmacytoid DCs (pDCs) and 
      myeloid DCs (mDCs) were investigated. Maturity status was assessed and surface 
      receptors with CTL-promoting potentials were studied. CD123(+) pDCs as well as 
      CD1c(+), CD141(+), and CD1c(-)CD141(-) mDCs were detected in tonsils and lymph 
      nodes. Both sites featured a similar presence of DC subsets, with CD123(+) pDC 
      being dominant and CD141(+) mDCs least frequent. Based on CD80/CD86 expression, 
      all DC subtypes featured a low degree of maturation. Expression of pattern 
      recognition receptors (PRRs) CD206, CD207, DC-SIGN, TLR2, and TLR4, as well as 
      the chemokine receptor XCR1, indicated DC subset-specific receptor profiles. We 
      conclude that tonsils and lymph nodes share common features in terms of DC subset 
      frequency and maturation as well as PRR and XCR1 expression pattern. Our work 
      suggests that both sites may be considered for vaccine deposition in DC-mediated 
      immunotherapy.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Askmyr, David
AU  - Askmyr D
AD  - Department of ORL, Head & Neck Surgery, Skane University Hospital, Lund, Sweden; 
      Department of Clinical Sciences, Lund University, Lund, Sweden. Electronic 
      address: david.askmyr@med.lu.se.
FAU - Abolhalaj, Milad
AU  - Abolhalaj M
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. Electronic 
      address: milad.abolhalaj@immun.lth.se.
FAU - Gomez Jimenez, David
AU  - Gomez Jimenez D
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. Electronic 
      address: david.gomez_jimenez@immun.lth.se.
FAU - Greiff, Lennart
AU  - Greiff L
AD  - Department of ORL, Head & Neck Surgery, Skane University Hospital, Lund, Sweden; 
      Department of Clinical Sciences, Lund University, Lund, Sweden. Electronic 
      address: lennart.greiff@skane.se.
FAU - Lindstedt, Malin
AU  - Lindstedt M
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. Electronic 
      address: malin.lindstedt@immun.lth.se.
FAU - Lundberg, Kristina
AU  - Lundberg K
AD  - Department of Immunotechnology, Lund University, Lund, Sweden. Electronic 
      address: kristina.lundberg@immun.lth.se.
LA  - eng
PT  - Journal Article
DEP - 20210909
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD80 protein, human)
RN  - 0 (CD86 protein, human)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Pattern Recognition)
RN  - 0 (XCR1 protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen/metabolism
MH  - CD40 Antigens/metabolism
MH  - Dendritic Cells/cytology/*metabolism
MH  - Female
MH  - Humans
MH  - Lymph Nodes/*cytology
MH  - Male
MH  - Middle Aged
MH  - Palatine Tonsil/*cytology
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - Receptors, Pattern Recognition/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Cancer
OT  - Dendritic cell
OT  - Immunotherapy
OT  - PRR
OT  - Vaccination
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/09/14 06:00
MHDA- 2022/03/03 06:00
CRDT- 2021/09/13 05:48
PHST- 2021/03/18 00:00 [received]
PHST- 2021/07/18 00:00 [revised]
PHST- 2021/08/10 00:00 [accepted]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/09/13 05:48 [entrez]
AID - S0198-8859(21)00206-8 [pii]
AID - 10.1016/j.humimm.2021.08.007 [doi]
PST - ppublish
SO  - Hum Immunol. 2021 Dec;82(12):976-981. doi: 10.1016/j.humimm.2021.08.007. Epub 
      2021 Sep 9.