PMID- 34512706
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210914
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Chromosome Painting Provides Insights Into the Genome Structure and Evolution of 
      Sugarcane.
PG  - 731664
LID - 10.3389/fpls.2021.731664 [doi]
LID - 731664
AB  - The genus Saccharum is composed of species with high polyploidy and highly varied 
      chromosome numbers, laying a challenge for uncovering its genomic structure and 
      evolution. We developed a chromosome 2 painting (CP2) probe by designing 
      oligonucleotides covering chromosome 2 of Saccharum spontaneum (2n = 8x = 64). 
      Fluorescence in situ hybridization (FISH) using this CP2 probe revealed six types 
      of ploidies from twenty S. spontaneum clones, including 6x, 8x, 10x, 11x, 12x, 
      and 13x clones. The finding of S. spontaneum clones with uneven of ploid 
      suggested that certain S. spontaneum clones come from hybridization. It renews 
      our knowledge that S. spontaneum is derived from autopolyploidization. Combined 
      with a S. spontaneum-specific probe, chromosome 2-derived chromosome or fragments 
      from either S. spontaneum or Saccharum officinarum can be identified in sugarcane 
      modern cultivars. We revealed unexpected high level of interspecific 
      recombination from introgressive S. spontaneum chromosomes (>50.0%) in cultivars 
      ROC22 and ZZ1, indicating frequent chromosome exchange in cultivars. 
      Intriguingly, we observed interspecific recombination recurring among either 
      homoeologous or non-homoeologous chromosomes in sugarcane cultivars. These 
      results demonstrated that chromosome painting FISH is a powerful tool in the 
      genome dissection of sugarcane and provide new insights into the genome structure 
      and evolution of the complex genus Saccharum.
CI  - Copyright (c) 2021 Meng, Wang, Khurshid, Raza, Han, Wang and Wang.
FAU - Meng, Zhuang
AU  - Meng Z
AD  - School of Life Sciences, Nantong University, Nantong, China.
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops (MOE), 
      Fujian Agriculture and Forestry University, Fuzhou, China.
FAU - Wang, Qinnan
AU  - Wang Q
AD  - Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou, China.
FAU - Khurshid, Haris
AU  - Khurshid H
AD  - Oilseeds Research Program, National Agricultural Research Centre, Islamabad, 
      Pakistan.
FAU - Raza, Ghulam
AU  - Raza G
AD  - Agricultural Biotechnology Division, National Institute for Biotechnology and 
      Genetic Engineering, Faisalabad, Pakistan.
FAU - Han, Jinlei
AU  - Han J
AD  - School of Life Sciences, Nantong University, Nantong, China.
FAU - Wang, Baohua
AU  - Wang B
AD  - School of Life Sciences, Nantong University, Nantong, China.
FAU - Wang, Kai
AU  - Wang K
AD  - School of Life Sciences, Nantong University, Nantong, China.
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops (MOE), 
      Fujian Agriculture and Forestry University, Fuzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210827
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8429501
OTO - NOTNLM
OT  - FISH
OT  - Saccharum
OT  - chromosome painting
OT  - interspecies recombination
OT  - ploidy
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/09/14 06:00
MHDA- 2021/09/14 06:01
PMCR- 2021/01/01
CRDT- 2021/09/13 06:44
PHST- 2021/06/27 00:00 [received]
PHST- 2021/08/10 00:00 [accepted]
PHST- 2021/09/13 06:44 [entrez]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2021/09/14 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.731664 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 Aug 27;12:731664. doi: 10.3389/fpls.2021.731664. 
      eCollection 2021.