PMID- 34512755
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210914
IS  - 1687-8450 (Print)
IS  - 1687-8469 (Electronic)
IS  - 1687-8450 (Linking)
VI  - 2021
DP  - 2021
TI  - Circular RNA hsa_circ_0006117 Facilitates Pancreatic Cancer Progression by 
      Regulating the miR-96-5p/KRAS/MAPK Signaling Pathway.
PG  - 9213205
LID - 10.1155/2021/9213205 [doi]
LID - 9213205
AB  - Circular RNAs (circRNAs) play key roles in many malignant tumors, including 
      pancreatic cancer (PC); however, whether circular RNA hsa_circ_0006117, a newly 
      identified circRNA, has a role in PC has not been investigated. Here, in order to 
      elucidate the role and potential molecular mechanisms of circRNAs, we utilized 
      bioinformatic tolls to screen the differentially expressed circRNAs in PC. 
      Subsequently, circular RNA hsa_circ_0006117 was identified as being highly 
      expressed in PC tissues in a screen of two GEO datasets, which was further 
      verified in PC cell lines and tissues. Then, its molecular characteristics were 
      investigated using methods such as Sanger sequencing and fluorescence in situ 
      hybridization (FISH). Functional experiments subsequently indicated that circular 
      RNA hsa_circ_0006117 facilitated the malignant behaviors of PC cells, prompting 
      that it plays an oncogenic role in PC. Moreover, we found that circular RNA 
      hsa_circ_0006117 exerts its PC-promoting effects via activating the 
      KRAS/mitogen-activated protein kinase (MAPK) signaling pathway. Through 
      bioinformatics exploration and dual-luciferase reporter assays, miR-96-5p was 
      identified as a downstream target of circular RNA hsa_circ_0006117. A series of 
      assays confirmed that circular RNA hsa_circ_0006117 acted as a miR-96-5p sponge, 
      thereby promoting the malignant features of PC in a miR-96-5p/KRAS axis-dependent 
      manner. Taken together, our study indicated, for the first time, that the 
      specifically highly expressed circular RNA hsa_circ_0006117 facilitates PC 
      progression via the modulation of the miR-96-5p/KRAS/MAPK signaling pathway and 
      might be a hopeful therapeutic target for PC.
CI  - Copyright (c) 2021 Tao Liu et al.
FAU - Liu, Tao
AU  - Liu T
AUID- ORCID: 0000-0002-1441-2765
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of 
      Guizhou Medical University, Guiyang 550000, China.
FAU - Zhou, Lei
AU  - Zhou L
AUID- ORCID: 0000-0003-4743-903X
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of 
      Guizhou Medical University, Guiyang 550000, China.
FAU - He, Zhiwei
AU  - He Z
AUID- ORCID: 0000-0002-5689-9006
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of 
      Guizhou Medical University, Guiyang 550000, China.
FAU - Chen, Yankun
AU  - Chen Y
AUID- ORCID: 0000-0003-0102-8833
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of 
      Guizhou Medical University, Guiyang 550000, China.
FAU - Jiang, Xueyi
AU  - Jiang X
AUID- ORCID: 0000-0002-5429-1865
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of 
      Guizhou Medical University, Guiyang 550000, China.
FAU - Xu, Jian
AU  - Xu J
AUID- ORCID: 0000-0002-9750-7689
AD  - Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 
      430060, China.
FAU - Jiang, Jianxin
AU  - Jiang J
AUID- ORCID: 0000-0001-7939-9082
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang 550000, China.
AD  - Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan 
      430060, China.
LA  - eng
PT  - Journal Article
DEP - 20210902
PL  - Egypt
TA  - J Oncol
JT  - Journal of oncology
JID - 101496537
PMC - PMC8433013
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this study.
EDAT- 2021/09/14 06:00
MHDA- 2021/09/14 06:01
PMCR- 2021/09/02
CRDT- 2021/09/13 06:45
PHST- 2021/06/10 00:00 [received]
PHST- 2021/08/05 00:00 [revised]
PHST- 2021/08/17 00:00 [accepted]
PHST- 2021/09/13 06:45 [entrez]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2021/09/14 06:01 [medline]
PHST- 2021/09/02 00:00 [pmc-release]
AID - 10.1155/2021/9213205 [doi]
PST - epublish
SO  - J Oncol. 2021 Sep 2;2021:9213205. doi: 10.1155/2021/9213205. eCollection 2021.