PMID- 34513340
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210914
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 9
DP  - 2021
TI  - Prediction of the mechanism of miRNAs in laryngeal squamous cell carcinoma based 
      on the miRNA-mRNA regulatory network.
PG  - e12075
LID - 10.7717/peerj.12075 [doi]
LID - e12075
AB  - In this study, a bioinformatics analysis is conducted to screen differentially 
      expressed miRNAs and mRNAs in laryngeal squamous cell carcinoma (LSCC). Based on 
      this information, we explored the possible roles of miRNAs in the pathogenesis of 
      LSCC. The RNA-Seq data from 79 laryngeal cancer samples in the Gene Expression 
      Omnibus (GEO) database were sorted. Differentially expressed miRNAs and mRNAs in 
      LSCC are screened using the PERL programming language, and it was analysed by 
      Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The 
      miRNA-mRNA regulatory network of LSCC is constructed using Cytoscape software. 
      Then, quantitative real-time PCR (QRT- PCR), Cell Counting Kit-8 (CCK8) and flow 
      cytometry analysis we are used to further validate key miRNAs. We identified 99 
      differentially expressed miRNAs and 2,758 differentially expressed mRNAs in LSCC 
      tissues from the GEO database. Four more important miRNAs displaying a high 
      degree of connectivity are selected, these results suggest that they play an 
      important role in the pathogenesis of LSCC. As shown in the present study, we 
      identified specific miRNA-mRNA networks associated with the occurrence and 
      development of LSCC through bioinformatics analysis. We found a miRNA molecule 
      closely related to LSCC based on miRNA-mRNA network: miR-140-3p was 
      down-regulated in LSCC. In addition, the potential antitumor effect of miR-140-3p 
      in LSCC was verified in the experiment, and it was proved that overexpression of 
      miR-140-3p could inhibit the proliferation of LSCC cells and promote cell 
      apoptosis, suggesting that miR-140-3p may be a potential tumor marker in LSCC.
CI  - (c)2021 Ma et al.
FAU - Ma, Jinhua
AU  - Ma J
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, China.
FAU - Hu, Xiaodong
AU  - Hu X
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, China.
FAU - Dai, Baoqiang
AU  - Dai B
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, China.
FAU - Wang, Qiang
AU  - Wang Q
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, China.
FAU - Wang, Hongqin
AU  - Wang H
AD  - Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210824
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC8395572
OTO - NOTNLM
OT  - Bioinformatics analysis
OT  - Laryngeal squamous cell carcinoma
OT  - miR-140-3p
OT  - miRNA-mRNA regulatory network
COIS- The authors declare there are no competing interests.
EDAT- 2021/09/14 06:00
MHDA- 2021/09/14 06:01
PMCR- 2021/08/24
CRDT- 2021/09/13 06:51
PHST- 2021/03/31 00:00 [received]
PHST- 2021/08/06 00:00 [accepted]
PHST- 2021/09/13 06:51 [entrez]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2021/09/14 06:01 [medline]
PHST- 2021/08/24 00:00 [pmc-release]
AID - 12075 [pii]
AID - 10.7717/peerj.12075 [doi]
PST - epublish
SO  - PeerJ. 2021 Aug 24;9:e12075. doi: 10.7717/peerj.12075. eCollection 2021.