PMID- 34514573
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20230422
IS  - 1309-5730 (Electronic)
IS  - 1018-5615 (Print)
IS  - 1018-5615 (Linking)
VI  - 38
IP  - 1
DP  - 2022
TI  - Detection of ALK Gene Rearrangements in Non-Small Cell Lung Cancer by 
      Immunocytochemistry and Fluorescence in Situ Hybridization on Cytologic Samples.
PG  - 16-24
LID - 10.5146/tjpath.2021.01542 [doi]
AB  - OBJECTIVE: Determination of the molecular status is mandatory for personalized 
      treatment of patients with non-small cell lung carcinoma. The present study was 
      performed to detect anaplastic lymphoma kinase (ALK) rearrangements in pulmonary 
      adenocarcinoma on cytology samples, using immunocytochemistry (ICC) and 
      fluorescence in situ hybridization (FISH) on cell-blocks to assess the diagnostic 
      reliability of these two techniques. MATERIAL AND METHOD: A total of 50 confirmed 
      lung adenocarcinoma cases were included. In all the 50 cases, ICC was performed 
      for ALK protein expression by using the D5F3 clone on Ventana platform. On the 
      basis of ALK protein expression on ICC, the cases were categorized as ALK 
      positive (2+ or 3+ strong cytoplasmic granular positivity) or negative (negative 
      or 1+ cytoplasmic granular positivity). FISH for detection of ALK gene 
      rearrangement was performed in 7 ALK ICC positive cases and 7 ALK ICC negative 
      cases using the Vysis ALK break apart FISH probe kit. RESULTS: Based on ICC, 
      7(14%) cases were ALK positive and 43(86%) were ALK negative. ALK gene 
      rearrangements in lung adenocarcinoma were more commonly seen in non-smokers 
      (31.25%) as compared to smokers (6.25%). Among the ALK-ICC positive cases, FISH 
      demonstrated break apart signal in 5 cases (ALK- ICC positive); however, no 
      break-apart signals were seen in 2 ALK-ICC positive and all the seven ALK-ICC 
      negative cases. CONCLUSION: Immunocytochemistry on cell- blocks using DF53 clone 
      is a highly sensitive and specific method for the detection of ALK gene 
      rearrangements in lung adenocarcinoma with a greater number of ALK positive cases 
      being detected on ICC as compared to the ALK-FISH.
FAU - Rachagiri, Suneel
AU  - Rachagiri S
AD  - Department of Pathology, Post Graduate Institute of Medical Education and 
      Research, CHANDIGARH, INDIA.
FAU - Gupta, Parikshaa
AU  - Gupta P
FAU - Gupta, Nalini
AU  - Gupta N
FAU - Rohilla, Manish
AU  - Rohilla M
FAU - Singh, Navneet
AU  - Singh N
FAU - Rajwanshi, Arvind
AU  - Rajwanshi A
LA  - eng
PT  - Journal Article
TT  - Detection of ALK Gene Rearrangements in Non-Small Cell Lung Cancer by 
      Immunocytochemistry and Fluorescence in Situ Hybridization on Cytologic Samples.
PL  - Turkey
TA  - Turk Patoloji Derg
JT  - Turk patoloji dergisi
JID - 9440471
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - *Adenocarcinoma/genetics/pathology
MH  - *Anaplastic Lymphoma Kinase/genetics
MH  - *Carcinoma, Non-Small-Cell Lung/genetics/pathology
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Lung Neoplasms/genetics/pathology
MH  - Receptor Protein-Tyrosine Kinases/genetics/metabolism
MH  - Reproducibility of Results
PMC - PMC9999692
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/14 06:00
MHDA- 2022/03/17 06:00
PMCR- 2022/01/21
CRDT- 2021/09/13 07:09
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
PHST- 2021/09/13 07:09 [entrez]
PHST- 2022/01/21 00:00 [pmc-release]
AID - 10.5146/tjpath.2021.01542 [doi]
PST - ppublish
SO  - Turk Patoloji Derg. 2022;38(1):16-24. doi: 10.5146/tjpath.2021.01542.