PMID- 34514810
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220531
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 30
IP  - 21
DP  - 2021 Nov 1
TI  - Effect of Hepatocyte Growth Factor-Transfected Human Umbilical Cord Mesenchymal 
      Stem Cells on Hepatic Stellate Cells by Regulating Transforming Growth 
      Factor-beta1/Smads Signaling Pathway.
PG  - 1070-1081
LID - 10.1089/scd.2021.0136 [doi]
AB  - Studies have shown that human umbilical cord mesenchymal stem cells (hUCMSCs) 
      could ameliorate liver fibrosis (LF) through inhibiting the activation of hepatic 
      stellate cells (HSCs). However, the specific mechanisms have not been studied 
      clearly. The purpose of this study was to explore the possible mechanism of 
      hepatocyte growth factor (HGF)-transfected hUCMSCs in inhibiting the 
      proliferation and activation of HSCs-T6. The upper and lower double-cell 
      coculture system was established among HGF-hUCMSCs, LV5-NC-hUCMSCs, hUCMSCs, and 
      HSCs-T6 in experimental groups; HSCs-T6 were cultured alone as control group. 
      After coculturing for 1, 2, and 3 days, results showed that HGF-transfected 
      hUCMSCs could decrease cell viability of HSCs-T6 and promote apoptosis; inhibit 
      their activation and reduce the expression of Collagen I, Collagen III, TGF-beta1, 
      Smad2 and Smad3, which may be related to inhibiting the activation of 
      TGF-beta1/Smads signaling pathway. These findings suggested that HGF-transfected 
      hUCMSCs may be used as an alternative and novel therapeutic approach for the 
      treatment of LF.
FAU - Yin, Fei
AU  - Yin F
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, Jilin Province, China.
FAU - Mao, Li-Cui
AU  - Mao LC
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, Jilin Province, China.
FAU - Cai, Qi-Qi
AU  - Cai QQ
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, Jilin Province, China.
FAU - Jiang, Wen-Hua
AU  - Jiang WH
AUID- ORCID: 0000-0002-9159-9869
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, Jilin Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211022
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cell Proliferation
MH  - *Hepatic Stellate Cells/metabolism
MH  - *Hepatocyte Growth Factor/administration & dosage/metabolism
MH  - Humans
MH  - Liver Cirrhosis/metabolism/therapy
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Signal Transduction
MH  - *Smad Proteins/metabolism
MH  - *Transforming Growth Factor beta1/metabolism
MH  - Umbilical Cord
OTO - NOTNLM
OT  - HGF
OT  - TGF-beta1/Smads signaling pathway
OT  - coculture
OT  - hepatic stellate cells-T6
OT  - human umbilical cord mesenchymal stem cells
OT  - liver fibrosis
EDAT- 2021/09/14 06:00
MHDA- 2022/03/22 06:00
CRDT- 2021/09/13 08:54
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/09/13 08:54 [entrez]
AID - 10.1089/scd.2021.0136 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2021 Nov 1;30(21):1070-1081. doi: 10.1089/scd.2021.0136. Epub 
      2021 Oct 22.