PMID- 34515141
OWN - NLM
STAT- MEDLINE
DCOM- 20220207
LR  - 20220207
IS  - 1423-0143 (Electronic)
IS  - 1420-4096 (Linking)
VI  - 46
IP  - 5
DP  - 2021
TI  - Measurement of Plasma Glucagon Levels Using Mass Spectrometry in Patients with 
      Type 2 Diabetes on Maintenance Hemodialysis.
PG  - 652-656
LID - 10.1159/000518027 [doi]
AB  - BACKGROUND: Recently, attention has been focused on the effect of glucagon on 
      blood glucose variability. The dynamics of glucagon have attracted attention as a 
      new target in the treatment of diabetes patients. However, the dynamics of 
      glucagon in hemodialysis (HD) patients with type 2 diabetes mellitus (T2DM) 
      remain unclear. OBJECTIVES: The aim of this study was to assess the dynamics of 
      glucagon in HD patients with T2DM. MATERIALS AND METHODS: We measured plasma 
      glucagon in HD patients with T2DM by liquid chromatography-high-resolution mass 
      spectrometry (LC-HRMS), sandwich enzyme-linked immunosorbent assay (ELISA), and 
      radioimmunoassay (RIA). The glucagon levels measured by each method were 
      compared. We used the glucagon levels determined by our developed LC-HRMS method 
      as the standard in this study. RESULTS: Plasma glucagon levels measured by 
      LC-HRMS before HD were significantly higher than those measured after HD. Plasma 
      glucagon levels measured using sandwich ELISA had a significantly higher 
      correlation with those measured using LC-HRMS compared with RIA. CONCLUSIONS: 
      This was the first study to assess glucagon levels in HD patients with T2DM using 
      LC-HRMS, which is considered a highly accurate method. Sandwich ELISA was shown 
      to measure glucagon levels accurately as well.
CI  - (c) 2021 The Author(s) Published by S. Karger AG, Basel.
FAU - Yoshizawa, Yuta
AU  - Yoshizawa Y
AD  - Department of Applied Molecular Medicine, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
AD  - Division of Clinical Nephrology and Rheumatology, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
FAU - Hosojima, Michihiro
AU  - Hosojima M
AD  - Department of Clinical Nutrition Science, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
FAU - Kabasawa, Hideyuki
AU  - Kabasawa H
AD  - Department of Clinical Nutrition Science, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
FAU - Tanabe, Naohito
AU  - Tanabe N
AD  - Department of Health and Nutrition, Faculty of Human Life Studies, University of 
      Niigata Prefecture, Niigata, Japan.
FAU - Miyachi, Atsushi
AU  - Miyachi A
AD  - Radioisotope and Chemical Analysis Center, Sanwa Kagaku Kenkyusho Co., Ltd., Mie, 
      Japan.
FAU - Hamajima, Hitoshi
AU  - Hamajima H
AD  - Radioisotope and Chemical Analysis Center, Sanwa Kagaku Kenkyusho Co., Ltd., Mie, 
      Japan.
FAU - Mieno, Eri
AU  - Mieno E
AD  - Radioisotope and Chemical Analysis Center, Sanwa Kagaku Kenkyusho Co., Ltd., Mie, 
      Japan.
FAU - Kobayashi, Masaki
AU  - Kobayashi M
AD  - Metabolic Signal Research Center, Institute for Molecular and Cellular 
      Regulation, Gunma University, Gunma, Japan.
FAU - Kitamura, Tadahiro
AU  - Kitamura T
AD  - Metabolic Signal Research Center, Institute for Molecular and Cellular 
      Regulation, Gunma University, Gunma, Japan.
FAU - Narita, Ichiei
AU  - Narita I
AD  - Division of Clinical Nephrology and Rheumatology, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
FAU - Saito, Akihiko
AU  - Saito A
AD  - Department of Applied Molecular Medicine, Kidney Research Center, Niigata 
      University Graduate School of Medical and Dental Sciences, Niigata, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210817
PL  - Switzerland
TA  - Kidney Blood Press Res
JT  - Kidney & blood pressure research
JID - 9610505
RN  - 9007-92-5 (Glucagon)
SB  - IM
MH  - Aged
MH  - Chromatography, Liquid
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Female
MH  - Glucagon/*blood
MH  - Humans
MH  - Male
MH  - Mass Spectrometry
MH  - Middle Aged
MH  - *Renal Dialysis
OTO - NOTNLM
OT  - Hemodialysis
OT  - Liquid chromatography-high-resolution mass spectrometry
OT  - Plasma glucagon
EDAT- 2021/09/14 06:00
MHDA- 2022/02/08 06:00
CRDT- 2021/09/13 09:20
PHST- 2021/04/13 00:00 [received]
PHST- 2021/06/21 00:00 [accepted]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2022/02/08 06:00 [medline]
PHST- 2021/09/13 09:20 [entrez]
AID - 000518027 [pii]
AID - 10.1159/000518027 [doi]
PST - ppublish
SO  - Kidney Blood Press Res. 2021;46(5):652-656. doi: 10.1159/000518027. Epub 2021 Aug 
      17.