PMID- 34515990
OWN - NLM
STAT- MEDLINE
DCOM- 20220125
LR  - 20240226
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 34
IP  - 6
DP  - 2021 Nov
TI  - Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma 
      via regulating YAP activity.
PG  - 1878-1887
LID - 10.1007/s13577-021-00606-z [doi]
AB  - Emerging evidence suggests that long non-coding RNA (lncRNA) is closely 
      associated with numerous human diseases, including cancer. However, the 
      functional relevance of lncRNA in human laryngeal squamous cell carcinoma (LSCC) 
      is largely unknown. In the current study, we described CCAT2, a previously 
      unappreciated oncogenic lncRNA in LSCC. CCAT2 was significantly upregulated in 
      human LSCC tissue and serum samples, associated with larger tumor volume, higher 
      clinical stage, and poorer differentiation status. Lentivirus-mediated CCAT2 
      knockdown notably repressed the cell viability, colony formation, and DNA 
      synthesis rate of LSCC. Screening of transcription factors revealed that YAP/TEAD 
      activity was affected by CCAT2 in LSCC cells. Further, CCAT2 directly binds to 
      YAP protein and blocks the phosphorylation of YAP induced by LATS1, resulting in 
      the nuclear translocation of YAP and the activation of YAP oncogenic targets, 
      such as CTGF, CYR61 and AMOTL2. Importantly, we also confirmed the regulation of 
      CCAT2 on YAP activity in vivo based on nude mice model. Altogether, we identified 
      a novel lncRNA that controls YAP nucleocytoplasmic shuttling and promotes LSCC 
      cell proliferation. Given the importance of YAP in tumorigenesis and progression, 
      our results provide insights to intervene LSCC by targeting the CCAT2/YAP axis.
CI  - (c) 2021. Japan Human Cell Society.
FAU - Zang, Yanzi
AU  - Zang Y
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
FAU - Li, Jing
AU  - Li J
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
FAU - Wan, Baoluo
AU  - Wan B
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
FAU - Tai, Yong
AU  - Tai Y
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
FAU - Liu, Hongjian
AU  - Liu H
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China. liuhjzz07@yeah.net.
FAU - Li, Qian
AU  - Li Q
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
FAU - Ji, Yuzi
AU  - Ji Y
AD  - Department of Otolaryngology, People's Hospital of Henan Province, 7 Weiwu Road, 
      Zhengzhou, 450003, Henan, People's Republic of China.
LA  - eng
GR  - 201201702196702196/henan province science and technology tackling project/
PT  - Journal Article
DEP - 20210913
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transcription Factors)
RN  - 0 (YY1AP1 protein, human)
RN  - 0 (long non-coding RNA CCAT2, human)
RN  - EC 2.7.1.- (LATS1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics/*pathology/therapy
MH  - Cell Cycle Proteins/*genetics/*metabolism
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - DNA, Neoplasm/metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/*pathology/therapy
MH  - Mice, Nude
MH  - Molecular Targeted Therapy
MH  - Phosphorylation/genetics
MH  - Protein Binding/genetics
MH  - Protein Serine-Threonine Kinases/physiology
MH  - RNA, Long Noncoding/genetics/metabolism/*physiology
MH  - Transcription Factors/*genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - Up-Regulation/genetics
MH  - Mice
OTO - NOTNLM
OT  - Biomarker
OT  - CCAT2
OT  - Protein binding
OT  - Translocation
OT  - YAP
EDAT- 2021/09/14 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/09/13 12:37
PHST- 2021/06/27 00:00 [received]
PHST- 2021/08/28 00:00 [accepted]
PHST- 2021/09/14 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/09/13 12:37 [entrez]
AID - 10.1007/s13577-021-00606-z [pii]
AID - 10.1007/s13577-021-00606-z [doi]
PST - ppublish
SO  - Hum Cell. 2021 Nov;34(6):1878-1887. doi: 10.1007/s13577-021-00606-z. Epub 2021 
      Sep 13.