PMID- 34518485
OWN - NLM
STAT- MEDLINE
DCOM- 20221019
LR  - 20221019
IS  - 1423-0399 (Electronic)
IS  - 0042-1138 (Linking)
VI  - 106
IP  - 10
DP  - 2022
TI  - Silencing of lncRNA MEG8 Represses the Viability, Migration, and Invasion of 
      Wilms' Tumor Cells through Mediating miR-23a-3p/CRK Axis.
PG  - 1075-1087
LID - 10.1159/000518502 [doi]
AB  - PURPOSE: Compelling evidence has unveiled the importance of long noncoding RNAs 
      (lncRNAs) in malignant behavior of Wilms' tumor (WT). Hereon, we intend to assess 
      the function and associated molecular mechanism of lncRNA maternally expressed 
      gene 8 (MEG8) in WT cells. METHODS: Expression levels of MEG8, miR-23a-3p, and 
      CT10 regulator of kinase (CRK) were determined by quantitative real-time 
      polymerase chain reaction. Cell viability was assessed by MTT assay. Besides, 
      wound healing assay and transwell assay were applied to examine abilities of cell 
      migration and invasion, respectively. Dual-luciferase reporter assay was employed 
      to test the interplay among MEG8, miR-23a-3p, and CRK. Western blot was used to 
      detect relative protein expression of CRK. RESULTS: MEG8 and CRK expression was 
      elevated, while miR-23a-3p expression was decreased in WT tissues and cells. The 
      histologic type, lymphatic metastasis, and National Wilms Tumor Study (NWTS) 
      stage were associated with the expression of MEG8, miR-23a-3p, and CRK in WT 
      patients. MEG8 knockdown or miR-23a-3p overexpression restrained WT cells in cell 
      viability, migration, and invasiveness in vitro. As to mechanism exploration, 
      MEG8 could directly bind to miR-23a-3p and then miR-23a-3p targeted CRK. MEG8 was 
      inversely correlated with miR-23a-3p and positively correlated with CRK in WT 
      tissues. Meantime, miR-23a-3p was inversely correlated with CRK in WT tissues. 
      Additionally, MEG8 knockdown-mediated suppressive impacts on cell viability, 
      migration, and invasiveness were reversed by overexpression of CRK or repression 
      of miR-23a-3p in WT cells. CONCLUSIONS: The cell viability, migration, and 
      invasiveness of WT cells were repressed by MEG8 knockdown via targeting the 
      miR-23a-3p/CRK axis.
CI  - (c) 2021 S. Karger AG, Basel.
FAU - Shen, Jing
AU  - Shen J
AD  - National Clinical Research Center for Child Health, The Children's Hospital, 
      Zhejiang University School of Medicine, Hangzhou City, China, 
      victor11272020@163.com.
FAU - Shu, Qiang
AU  - Shu Q
AD  - National Clinical Research Center for Child Health, The Children's Hospital, 
      Zhejiang University School of Medicine, Hangzhou City, China.
LA  - eng
PT  - Journal Article
DEP - 20210908
PL  - Switzerland
TA  - Urol Int
JT  - Urologia internationalis
JID - 0417373
RN  - 0 (CRK protein, human)
RN  - 0 (MIRN23a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-crk)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Kidney Neoplasms/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Proto-Oncogene Proteins c-crk/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Wilms Tumor/genetics
OTO - NOTNLM
OT  - CT10 regulator of kinase
OT  - Long noncoding RNA
OT  - Maternally expressed gene 8
OT  - Wilms' tumor
OT  - miR-23a-3p
EDAT- 2021/09/15 06:00
MHDA- 2022/10/20 06:00
CRDT- 2021/09/14 05:58
PHST- 2021/01/11 00:00 [received]
PHST- 2021/07/14 00:00 [accepted]
PHST- 2021/09/15 06:00 [pubmed]
PHST- 2022/10/20 06:00 [medline]
PHST- 2021/09/14 05:58 [entrez]
AID - 000518502 [pii]
AID - 10.1159/000518502 [doi]
PST - ppublish
SO  - Urol Int. 2022;106(10):1075-1087. doi: 10.1159/000518502. Epub 2021 Sep 8.