PMID- 34519979
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2436
DP  - 2022
TI  - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier 
      Tablets in Bioreactor.
PG  - 113-125
LID - 10.1007/7651_2021_425 [doi]
AB  - Mesenchymal stem cells show great potential in tissue engineering and cell-based 
      therapies. This protocol demonstrates the use of 3D TableTrix((R)) microcarrier 
      tablets for large-scale manufacturing of human umbilical cord mesenchymal stem 
      cells (hUCMSCs) in a 5-L stirred tank bioreactor. 3D TableTrix((R)) microcarrier 
      tablets readily disperse into tens of thousands of porous microcarriers to 
      simplify cell seeding, and 3D FloTrix((R)) vivaSPIN bioreactor could automate, 
      monitor, and control the entire culture process. 3D TableTrix((R)) microcarriers 
      could also be fully dissolved upon adding dissolution reagent to gently harvest 
      the expanded cells at a high recovery rate. With this protocol, more than 10(9) 
      cells could be produced in a 5-L bioreactor.
CI  - (c) 2021. Springer Science+Business Media, LLC.
FAU - Xu, Huanye
AU  - Xu H
AD  - Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China.
FAU - Cong, Zhongxiao
AU  - Cong Z
AD  - Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China.
FAU - Zhang, Yuanyuan
AU  - Zhang Y
AD  - Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China.
FAU - Yan, Xiaojun
AU  - Yan X
AD  - Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China. 
      yanxiaojun@cytoniche.com.
FAU - Du, Yanan
AU  - Du Y
AD  - Department of Biomedical Engineering, School of Medicine, Tsinghua-PKU Center for 
      Life Sciences, Tsinghua University, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Tablets)
SB  - IM
MH  - Bioreactors
MH  - *Cell Culture Techniques/methods
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Tablets
MH  - Umbilical Cord
OTO - NOTNLM
OT  - 3D cell culture
OT  - Bioreactor
OT  - Dissolvable microcarrier
OT  - Large-scale expansion
OT  - Mesenchymal stem cells
EDAT- 2021/09/15 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/09/14 13:22
PHST- 2021/09/15 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/09/14 13:22 [entrez]
AID - 10.1007/7651_2021_425 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2436:113-125. doi: 10.1007/7651_2021_425.