PMID- 34520009
OWN - NLM
STAT- MEDLINE
DCOM- 20220105
LR  - 20220105
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2373
DP  - 2022
TI  - A Human Neurovascular Unit On-a-Chip.
PG  - 107-119
LID - 10.1007/978-1-0716-1693-2_7 [doi]
AB  - Protection of the central nervous system (CNS) and cerebral homeostasis depend 
      upon the blood-brain barrier (BBB) functions and permeability. BBB restrictive 
      permeability hinders drug delivery for the treatment of several neurodegenerative 
      diseases and brain tumors. Several in vivo animal models and in vitro systems 
      have been developed to understand the BBB complex mechanisms and aid in the 
      design of improved therapeutic strategies. However, there are still many 
      limitations that should be addressed to achieve the structural and chemical 
      environment of a human BBB. We developed a microfluidic-based model of the 
      neurovascular unit. A monolayer of human cerebral endothelial cells (hCMEC-D3) 
      was grown and cocultured with human brain microvascular pericytes (hBMVPC), and 
      human induced pluripotent stem cells differentiated into astrocytes (hiPSC-AC) 
      and neurons (hiPSC-N). To visualize the physiological morphology of each cell 
      type, we used fluorescent cell-specific markers and confocal microscopy. 
      Permeation of fluorescent solutes with different molecular weights was measured 
      to demonstrate that the developed BBB was selectively permeable as a functional 
      barrier.
CI  - (c) 2022. Springer Science+Business Media, LLC, part of Springer Nature.
FAU - Lee, Sharon Wei Ling
AU  - Lee SWL
AD  - Singapore Immunology Network (SIgN), Biomedical Sciences Institute, Agency for 
      Science, Technology, and Research (A*STAR), Singapore, Singapore.
AD  - Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, 
      National University of Singapore (NUS), Singapore, Singapore.
FAU - Rogosic, Renato
AU  - Rogosic R
AD  - Sensor Engineering, Faculty of Science and Engineering, Maastricht University, 
      Maastricht, Netherlands.
FAU - Venturi, Claudia
AU  - Venturi C
AD  - Department of Chemistry, Materials and Chemical Engineering (CMIC) "Giulio 
      Natta", Politecnico di Milano, Milan, Italy.
FAU - Raimondi, Manuela Teresa
AU  - Raimondi MT
AD  - Department of Chemistry, Materials and Chemical Engineering (CMIC) "Giulio 
      Natta", Politecnico di Milano, Milan, Italy.
FAU - Pavesi, Andrea
AU  - Pavesi A
AD  - Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology, 
      and Research (A*STAR), Singapore, Singapore.
FAU - Adriani, Giulia
AU  - Adriani G
AD  - Singapore Immunology Network (SIgN), Biomedical Sciences Institute, Agency for 
      Science, Technology, and Research (A*STAR), Singapore, Singapore. 
      giulia_adriani@immunol.a-star.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier
MH  - Coculture Techniques
MH  - Endothelial Cells
MH  - Humans
MH  - Induced Pluripotent Stem Cells
MH  - *Lab-On-A-Chip Devices
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - Human induced pluripotent stem cells
OT  - Microfluidic
OT  - Neurovascular unit
OT  - Organotypic model
EDAT- 2021/09/15 06:00
MHDA- 2022/01/06 06:00
CRDT- 2021/09/14 13:23
PHST- 2021/09/14 13:23 [entrez]
PHST- 2021/09/15 06:00 [pubmed]
PHST- 2022/01/06 06:00 [medline]
AID - 10.1007/978-1-0716-1693-2_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2373:107-119. doi: 10.1007/978-1-0716-1693-2_7.