PMID- 34521024 OWN - NLM STAT- MEDLINE DCOM- 20220208 LR - 20220208 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 100 DP - 2021 Nov TI - Baicalin protects against zearalenone-induced chicks liver and kidney injury by inhibiting expression of oxidative stress, inflammatory cytokines and caspase signaling pathway. PG - 108097 LID - S1567-5769(21)00733-5 [pii] LID - 10.1016/j.intimp.2021.108097 [doi] AB - Zearalenone (ZEA) is a secondary metabolite produced by fungi such as Fusarium and Fusarium flavum, which is classified as a mycotoxin. Crops and feed in a humid surrounding are widely polluted by ZEA, which further endangering the healthful aquaculture of poultry and even human health. Up to now, prevention and cure of mycotoxicosis is still a crucial subject of poultry husbandry. Baicalin (BAI) is a flavonoid refined from dried roots of Scutellaria baicalensis possessing the function of hepatoprotective, anti-inflammatory, anti-oxidant, and anti-atherosclerotic efficacies.etc. But whether Baicalin also has a protective effect against ZEA intoxication is unclear. Therefore, the aim of this study was to establish a model of ZEA-induced toxic injury in chicks, and then to investigate the way in which Baicalin plays a protective role in the mechanism of ZEA-induced liver and kidney injury in chicks. The results exhibit that Baicalin could not only significantly decrease aspartate aminotransferase (AST) , alanine aminotransferase (ALT) and creatinine (Cre) levels in serum, but also ameliorate ZEA-induced pathologic changes of liver and kidney. Baicalin could also significantly regulate ZEA-induced the changes of catalase (CAT) , malondialdehyde (MDA) , total sulfhydryl group , except for glutathione peroxidase (GSH-px) , and inhibit the mRNA levels of inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) , interleukin-1beta (IL-1beta) and cyclooxygenase-2 (COX-2) with caspase-3 and caspase-11 in the caspase signaling pathway , meanwhile inhibit the cell apoptosis in immunohistochemistry. In summary, we successfully established a model of ZEA-induced liver injury in chicks, and confirm that Baicalin can reduce ZEA-induced liver and kidney injury in chicks. The mechanism of these effects is via inhibiting inflammation, oxidative stress and apoptosis, which also indicates the potential applicability of Baicalin for the prevention and treatment of ZEA-induced toxicity in chicks. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Xu, Jingnan AU - Xu J AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Li, Shurou AU - Li S AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Jiang, Liqiang AU - Jiang L AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Gao, Xinxin AU - Gao X AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Liu, Wei AU - Liu W AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Zhu, Xingyi AU - Zhu X AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Huang, Wenlong AU - Huang W AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Zhao, Haiguang AU - Zhao H AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Wei, Zhengkai AU - Wei Z AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. FAU - Wang, Kai AU - Wang K AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. Electronic address: wkwkwky@126.com. FAU - Yang, Zhengtao AU - Yang Z AD - College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China. Electronic address: yangzhengtao01@sina.com. LA - eng PT - Journal Article DEP - 20210911 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Antioxidants) RN - 0 (Cytokines) RN - 0 (Flavonoids) RN - 0 (Inflammation Mediators) RN - 347Q89U4M5 (baicalin) RN - 5W827M159J (Zearalenone) RN - EC 3.4.22.- (Caspases) SB - IM MH - Acute Kidney Injury/enzymology/immunology/pathology/*prevention & control MH - Animals MH - Anti-Inflammatory Agents/*pharmacology MH - Antioxidants/*pharmacology MH - Apoptosis/*drug effects MH - Caspases/genetics/*metabolism MH - Chemical and Drug Induced Liver Injury/enzymology/immunology/pathology/*prevention & control MH - Chickens MH - Cytokines/genetics/*metabolism MH - Disease Models, Animal MH - Flavonoids/*pharmacology MH - Inflammation Mediators/*metabolism MH - Kidney/*drug effects/immunology/metabolism/pathology MH - Liver/*drug effects/enzymology/immunology/pathology MH - Oxidative Stress/*drug effects MH - Signal Transduction MH - Zearalenone OTO - NOTNLM OT - Baicalin OT - Chicks OT - Kidney injury OT - Liver Injury OT - Zearalenone EDAT- 2021/09/15 06:00 MHDA- 2022/02/09 06:00 CRDT- 2021/09/14 20:21 PHST- 2021/05/15 00:00 [received] PHST- 2021/08/20 00:00 [revised] PHST- 2021/08/26 00:00 [accepted] PHST- 2021/09/15 06:00 [pubmed] PHST- 2022/02/09 06:00 [medline] PHST- 2021/09/14 20:21 [entrez] AID - S1567-5769(21)00733-5 [pii] AID - 10.1016/j.intimp.2021.108097 [doi] PST - ppublish SO - Int Immunopharmacol. 2021 Nov;100:108097. doi: 10.1016/j.intimp.2021.108097. Epub 2021 Sep 11.