PMID- 34522245
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220426
IS  - 1734-1922 (Print)
IS  - 1896-9151 (Electronic)
IS  - 1734-1922 (Linking)
VI  - 17
IP  - 5
DP  - 2021
TI  - Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding 
      RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma.
PG  - 1164-1174
LID - 10.5114/aoms.2019.91352 [doi]
AB  - INTRODUCTION: This study was performed to explore the function of B-Raf 
      proto-oncogene-activated non-coding RNA (BANCR) in laryngeal squamous cell 
      carcinoma (LSCC) and cisplatin resistance. MATERIAL AND METHODS: The relative 
      expression level of long non-coding RNA (lncRNA) BANCR was examined by qRT-PCR in 
      tumor tissues and adjacent tissues, normal laryngeal cells (Het-1A) and laryngeal 
      squamous carcinoma cells (TU686, TU177). Cisplatin-resistant laryngeal squamous 
      carcinoma cell lines (TU686-DDP-R, TU177-DDP-R) were established. Next, we 
      inhibited BANCR expression by transfecting siRNA-BANCR and enhanced BANCR 
      expression by transfecting pcDNA3.1-BANCR into TU686, TU177, TU686-DDP-R and 
      TU177-DDP-R cells. The CCK-8 assay and clone formation assay were performed to 
      detect colony proliferation ability and formation ability of cells. Further, to 
      investigate through which BANCR cell viability/formation is regulated, we 
      detected the expression of MRP1, Bcl-2, p-PKB, and Bax by western blot. RESULTS: 
      BANCR was highly expressed in laryngeal squamous carcinoma tissues and cells. 
      Chemoresistance was generated in TU686-DDP-R and TU177-DDP-R compared with TU686 
      and TU177 cells after cisplatin treatment. In addition, upregulated lncRNA BANCR 
      reduced or postponed cell sensitivity to cisplatin by enhancing cell 
      proliferation in TU686 and TU177 cells. Meanwhile, the expression of MRP1, Bcl-2, 
      and p-PKB was increased, while Bax was reduced. After cisplatin treatment, 
      down-regulation of BANCR could consequently attenuate TU686-DDP-R and TU177-DDP-R 
      cell proliferation, and the expression of MRP1, Bcl-2, and p-PKB was decreased 
      and Bax was increased. CONCLUSIONS: Down-regulation of BANCR reverses cisplatin 
      resistance of cisplatin-resistant LSCC cell lines.
CI  - Copyright: (c) 2019 Termedia & Banach.
FAU - Han, Weiwei
AU  - Han W
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine 
      Centre, Tianjin, China.
FAU - Niu, Lin
AU  - Niu L
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine 
      Centre, Tianjin, China.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine 
      Centre, Tianjin, China.
FAU - Liu, Jixiang
AU  - Liu J
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine 
      Centre, Tianjin, China.
FAU - Li, Huanying
AU  - Li H
AD  - Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine 
      Centre, Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20191231
PL  - Poland
TA  - Arch Med Sci
JT  - Archives of medical science : AMS
JID - 101258257
PMC - PMC8425235
OTO - NOTNLM
OT  - B-Raf proto-oncogene-activated non-coding RNA
OT  - cisplatin
OT  - laryngeal squamous cell carcinoma
OT  - resistance
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/31 00:00
MHDA- 2019/12/31 00:01
PMCR- 2019/12/31
CRDT- 2021/09/15 07:15
PHST- 2018/09/29 00:00 [received]
PHST- 2018/10/14 00:00 [accepted]
PHST- 2021/09/15 07:15 [entrez]
PHST- 2019/12/31 00:00 [pubmed]
PHST- 2019/12/31 00:01 [medline]
PHST- 2019/12/31 00:00 [pmc-release]
AID - 98949 [pii]
AID - 10.5114/aoms.2019.91352 [doi]
PST - epublish
SO  - Arch Med Sci. 2019 Dec 31;17(5):1164-1174. doi: 10.5114/aoms.2019.91352. 
      eCollection 2021.