PMID- 34522723
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220426
IS  - 2352-3204 (Electronic)
IS  - 2352-3204 (Linking)
VI  - 18
DP  - 2021 Dec
TI  - Repair of spinal cord injury in rats via exosomes from bone mesenchymal stem 
      cells requires sonic hedgehog.
PG  - 309-315
LID - 10.1016/j.reth.2021.08.007 [doi]
AB  - OBJECTIVE: The loss of neural ability leading to subsequent diminishing of motor 
      function and the impairment below the location of the injury is a result of the 
      SCI (Spinal Cord Injury). Among the many therapeutic agents for SCI, the exosomes 
      considered as extracellular vesicles seem to be the most promising. Sonic 
      Hedgehog (Shh) is an exosome-carrying protein. This Study's purpose was to 
      identify whether Shh is required for exosomes from BMSCs (mesenchymal stem cells 
      of the bone) and plays a protective effect on SCI. METHODS: Spinal cord injection 
      with shRNA Shh-adeno associated virus (sh-Shh-AAV) were used to silence Shh. 
      Exosomes were extracted from BMSCs. Rats that had suffered SCI were given 
      intravenous injections of exosomes through the veins of the tail. 
      Immunohistochemistry was used to identify the expression of Shh glycoprotein 
      molecule as well as the expression of Gli-1 (glioma-associated oncogene homolog 
      1) in the rat spinal cord tissues. Western blot was performed to measure the 
      levels of growth associated protein-43 (GAP-43). The BBB (Basso Beattie 
      Bresnahan) score was used to assess the motor functions of the hind legs. In the 
      same manner, terminal deoxynucleotidyl transferase-mediated dUTP nick-end 
      labeling or TUNEL and Nissl Staining was deployed to assess the level of 
      regeneration of neurons and assess the level of histopathological damage in the 
      tissues of the Spinal Cord. RESULTS: In the case of the rats with SCI, the levels 
      of display of Gli-1 and Shh showed dramatic improvement after the BMSCs exosome 
      injections. In comparison to rats with SCI, the subjects of BMSCs exosomes group 
      showed an improvement in their SCI, including a higher BBB score and Nissl body 
      count, increasing GAP-43 expression, along with a much-decreased number of cells 
      that suffered apoptosis. While the exosome effect on Spinal Cord Injury was 
      completely ineffective in rats that had Shh silencing. CONCLUSIONS: Exosomes 
      secreted from BMSCs showed great effectiveness in the SCI healing with a vital 
      involvement of Shh in this repair.
CI  - (c) 2021 The Japanese Society for Regenerative Medicine. Production and hosting by 
      Elsevier B.V.
FAU - Jia, Yijia
AU  - Jia Y
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Yang, Jianwen
AU  - Yang J
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Lu, Tingsheng
AU  - Lu T
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Pu, Xingwei
AU  - Pu X
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Chen, Qiling
AU  - Chen Q
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Ji, Linsong
AU  - Ji L
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
FAU - Luo, Chunshan
AU  - Luo C
AD  - Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 
      550002, China.
LA  - eng
PT  - Journal Article
DEP - 20210901
PL  - Netherlands
TA  - Regen Ther
JT  - Regenerative therapy
JID - 101709085
PMC - PMC8416644
OTO - NOTNLM
OT  - BMSCs
OT  - Exosomes
OT  - Sonic hedgehog
OT  - Spinal cord injury
COIS- The authors declare that they have no conflict of interest.
EDAT- 2021/09/16 06:00
MHDA- 2021/09/16 06:01
PMCR- 2021/09/01
CRDT- 2021/09/15 07:19
PHST- 2021/05/12 00:00 [received]
PHST- 2021/07/24 00:00 [revised]
PHST- 2021/08/16 00:00 [accepted]
PHST- 2021/09/15 07:19 [entrez]
PHST- 2021/09/16 06:00 [pubmed]
PHST- 2021/09/16 06:01 [medline]
PHST- 2021/09/01 00:00 [pmc-release]
AID - S2352-3204(21)00066-3 [pii]
AID - 10.1016/j.reth.2021.08.007 [doi]
PST - epublish
SO  - Regen Ther. 2021 Sep 1;18:309-315. doi: 10.1016/j.reth.2021.08.007. eCollection 
      2021 Dec.